糖肾方对db/db小鼠肾脏氧化损伤的保护作用  

Protective effect of Tangshen Formula on oxidative stress in kidney of db/db mice

作　　者：张并璇[1,2] 孔勤[2] 张浩军[2] 赵婷婷[2] 严美花[2] 赵海玲[2] 李平[1,2] 

机构地区：[1]北京中医药大学,北京100029 [2]中日友好医院,北京100029

出　　处：《中华中医药杂志》2016年第6期2150-2153,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：科技部国际科技合作项目(No.2011DFA31860);国家自然科学基金项目(No.81173422;No.81130066;No.81473526);北京市自然科学基金项目(No.7142143)~~

摘　　要：目的:观察糖肾方对C57BLKS/J db/db(db/db)小鼠肾脏损伤的保护作用,并探讨其机制。方法:8周龄雄性自发性2型糖尿病db/db小鼠与同源不发病db/m小鼠,随机分为正常对照组(db/m组)、模型组(db/db组)、糖肾方给药组(db/db+TSF组),连续喂养12周,记录体质量、肾重、血糖及24h尿白蛋白水平,观察肾脏病理改变,并检测肾组织NADPH氧化酶及Nrf2信号通路表达。结果:与db/m组相比,db/db组小鼠体质量、肾重增加,血糖升高,24h尿白蛋白维持在高水平(P<0.01),肾小球系膜基质增生;糖肾方干预后可降低db/db小鼠体质量、肾重及24h尿白蛋白水平(P<0.05,P<0.01),改善肾组织病理改变,下调肾皮质Nox2、Nox4、p22phox、Nrf2、NQO1表达(P<0.05,P<0.01),上调HO-1表达(P<0.05)。结论:糖肾方对db/db小鼠肾脏具有保护作用,其机制可能与下调NADPH氧化酶表达从而改善氧化损伤有关。Objective： To observe the renoprotective effect and mechanisms of Tangshen Formula（TSF） on db/db mice. Methods： Eight-week-old male db/db mice and homology db/m mice with spontaneously type 2 diabetes mellitus were divided into normal control group（db/m group）, model group（db/db group） and TSF group（db/db＋TSF group） and fed for 12 weeks. Body weights, kidney weights, blood glucose levels, 24 h urine protein levels, morphology study and oxidative stress markers were detected. The expression of NADPH oxidase and Nrf2 signal path were detected. Results： Compared with db/m group, the db/db group showed higher body and kidney weights, higher blood glucose levels, higher 24 h urine protein levels（P〈0.01） and severe morphological changes; TSF treatment decreased body weights, kidney weights and 24 h urine protein levels（P〈0.05, P〈0.01）, alleviated morphological changes, suppressed the expression of Nox2, Nox4, p22 phox, Nrf2, NQO1（P〈0.05, P〈0.01）, upregulated HO-1 expression（P〈0.05）. Conclusion： TSF has renoprotective effect on db/db mice, and the mechanism might be related with reducing the expression of NADPH oxidase to suppress oxidative stress.

关 键 词：糖肾方 糖尿病肾病 氧化应激 NADPH氧化酶 核因子E2相关因子2 

分 类 号：R285.5[医药卫生—中药学]