芪冬活血饮药物血清对脂多糖刺激下人肺微血管内皮细胞E-selectin和IP-10表达的影响  

Effects of Qidong Huoxue Drink containing serum on expression of E-selectin and IP-10 in LPS-induced human pulmonary microvascular endothelial cells

作　　者：马春芳[1] 王寅[1] 孟丽[1] 蔡宛如[1] 

机构地区：[1]浙江中医药大学附属第二医院,浙江省新华医院,杭州310005

出　　处：《中华中医药杂志》2016年第6期2251-2253,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金面上项目(No.81273678)~~

摘　　要：目的:研究芪冬活血饮药物血清对人肺微血管内皮细胞(HPMEC)表达E-selectin和IP-10的影响。方法:将细胞随机分为5组,空白组,LPS组,正常大鼠血清+LPS组,5%药物血清+LPS组,10%药物血清+LPS组,用不同浓度的药物血清培养HPMEC 4h后,再加入1μg/mL LPS刺激HPMEC,用RT-PCR方法检测E-selectin和IP-10的mRNA表达,ELISA法检测细胞上清液中E-selectin和IP-10蛋白水平。结果:LPS刺激HPMEC细胞后E-selectin和IP-10的表达和分泌显著高于空白对照(P<0.01),而芪冬活血饮药物血清则能降低该类细胞因子的表达和释放(P<0.05)。结论:芪冬活血饮药物血清能明显抑制HPMEC的活化,降低E-selectin和IP-10的释放,减轻炎性反应损伤。Objective： To investigate the effects of Qidong Huoxue Drink containing serum on expression of E-selectin and IP-10 in LPS-induced human pulmonary microvascular endothelial cells（HPMEC）. Methods： The cells were randomly divided into five groups： blank group, LPS group, Normal rat serum ＋ LPS group, 5% Qidong Huoxue Drink-containing serum ＋ LPS group, 10% Qidong Huoxue Drink-containing serum ＋ LPS group. All cells were pretreated with different concentration of Qidong Huoxue Drink-containing serum for 4h, and then pipetted 1μg/ml LPS to induce the cells. The gene and protein expression of E-selectin and IP-10 were detected with RT-PCR and ELISA assay, respectively. Results： Compared with the blank group, the expression of E-selectin and IP-10 in LPS group were increased（P0.01）, which could be suppressed by different concentration of Qidong Huoxue Yin containing serum（P0.05）. Conclusion： Qidong Huoxue Drink containing serum could reduce the release of E-selectin and IP-10, inhibiting the activation of HPMEC and decreasing an inflammation injury.

关 键 词：芪冬活血饮 药物血清 人肺微血管内皮细胞 细胞因子 

分 类 号：R285.5[医药卫生—中药学]