脂肪酶Candida sp.99-125催化羟基酸甲酯缩聚合成脂肪族聚酯  

Synthesis of Aliphatic Polyesters via Methyl Hydroxy Acid Polycondensation Catalyzed by Lipase Candida sp. 99-125

作　　者：孙芹[1] 方正[1] 郭凯[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211816

出　　处：《高校化学工程学报》2016年第3期655-660,共6页Journal of Chemical Engineering of Chinese Universities

基　　金：国家自然科学基金项目(U1463201;21402240);中国国家重点基础研究项目(973项目2012CB725204)

摘　　要：采用游离脂肪酶Candida sp.99-125为催化剂,无溶剂体系中成功地将几种羟基酸酯转化为脂肪族聚酯。选取单体6-羟基己酸甲酯作为缩聚反应模板体系,考察了包括酶量、单体纯度、温度和表面活性剂等条件对反应的影响。结果表明,纯度大于95%的单体即可在无溶剂条件下合成脂肪酶族聚酯。吐温40和吐温80有使分子量分布变窄和提高重均分子量的能力。脂肪酶催化剂重复利用能力优异,经5批次反应后,其相对活性保持在90%以上。在最优条件下,脂肪酶对长链单体表现出很高的选择性。通过改变单体的碳链长度,可使脂肪族聚酯的重均分子量从3200到10000。Several hydroxyl acid esters were successfully converted into aliphatic polyesters catalyzed by free lipase Candida sp.99-125 without organic solvents. The polycondensation of methyl 6-hydroxycaproate was selected as a model process in this study. The effects of different conditions including catalyst dosage, monomer purity, temperature and surfactant on the reaction were studied. The results show that aliphatic polyesters can be synthesized with minimum monomer purity of 95% under solvent-free conditions. Tween 40 and Tween 80 can narrow molecular weight distribution and increase weight-average molecular weight. Moreover, the enzyme maintains activity up to five rounds, and lipase shows a high selectivity for monomers with long chain under the optimal conditions. The weight-average molecular weight can be adjusted in a range of 3200 to 10000 Da by changing monomer carbon chain length.

关 键 词：脂肪酶Candida sp.99-125 6-羟基己酸甲酯 缩聚 无溶剂 

分 类 号：TQ323[化学工程—合成树脂塑料工业] O643.3[理学—物理化学]