酸性红B高效降解耐盐菌株BY-2的分离鉴定及降解特性研究  

Isolation and Identification of a Salt-Tolerant Bacteria Strain BY-2 for Acid Red B Degradation

作　　者：杨阳[1] 胡翔[1] 

机构地区：[1]北京化工大学化学工程学院,北京100029

出　　处：《高校化学工程学报》2016年第3期734-740,共7页Journal of Chemical Engineering of Chinese Universities

基　　金：国家科技支撑计划(2011BAE07B09-2)

摘　　要：从已运行一年的三相内循环好氧生物流化床系统中分离筛选出一株降解酸性红B的高效耐盐菌株BY-2,通过对菌株的理化形态特征及16s r RNA序列分析,初步确定菌株BY-2为Pseudomonas sp.属。考察了初始pH、盐度、染料浓度以及接种量等条件对酸性红B降解与菌体生长的影响。结果表明:初始pH为7,盐浓度为50 g×L^(-1),酸性红B初始浓度为500 mg×L^(-1),接种量2%,反应18 h时,酸性红B的降解率可达90%以上。降解中间产物的紫外-可见光光谱和GC-MS分析说明菌株BY-2对酸性红B有良好的降解作用,菌株BY-2在实际染料废水处理中具有潜在应用价值。A salt-tolerant bacteria strain BY-2 for Acid Red B（ARB） degradation was isolated from a three-phase interna1-circulation aerobic bio-fluidized bed after one year running. The strain was identified as Pseudomonas sp based on physico-biochemical characteristics and 16 s r RNA gene analysis. The effects of different conditions, such as initial pH, salinity, dye concentration and inoculum ratio on ARB degradation and cell growth were evaluated. The experimental results show that the ARB degradation efficiency is up to 90% when pH, salt concentration, initial ARB concentration, inoculum ratio and reaction time are 7, 50 g×L^-1, 500 mg×L^-1, 2% and 18 h respectively. UV-V is absorption spectra and GC-MS results of degradation intermediates indicate that the strain BY-2 show good degradation performance, which can be used in dye wastewater treatments.

关 键 词：偶氮染料 酸性红B 脱色 假单胞菌属 

分 类 号：Q813.11[生物学—生物工程]