中药筋脉通对糖尿病大鼠背根神经节神经元超微结构影响的研究  

Effects of Jinmaitong on ultrastructure of DRG neurons in STZ-DM rats

作　　者：杨丹[1] 梁晓春[1] 屈岭[1] 吴群励[1] 吴亚楠[1] 石玥[1] 戴威[2] 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院中医科转化医学中心,100730 [2]中国医学科学院基础医学研究所电镜室

出　　处：《环球中医药》2016年第6期654-658,共5页Global Traditional Chinese Medicine

基　　金：北京市自然科学基金(7122147)

摘　　要：目的观察中药筋脉通对链尿佐菌素(streptozotocin,STZ)诱导的糖尿病(diabetes mellitus,DM)大鼠背根神经节(dorsal root ganglia,DRG)感觉神经元超微结构的影响。方法雄性SD大鼠18只,腹腔内注射STZ诱导建立糖尿病大鼠模型,随机分为糖尿病组、筋脉通组与牛磺酸组,每组6只大鼠,并设正常对照组6只。成模后每天1次灌胃给药,持续16周。药物干预16周后,用电子Von Frey仪检测机械痛阈值,取大鼠背根神经节,按常规方法制成切片后,在电镜下观察背根神经节神经元超微结构的病理学改变。结果糖尿病大鼠的机械痛阈值较正常组显著明显下降(P<0.01),背根神经节感觉神经元超微结构出现重度损伤,神经元细胞中线粒体数量显著减少(P<0.01),并且线粒体肿胀及空泡化比率、凋亡率显著升高(P<0.01);中药筋脉通可显著提高机械痛阈值(P<0.01),减轻神经元的损伤,提高神经元中线粒体的数量(P<0.05),并且降低线粒体的肿胀及空泡化比率及凋亡率(P<0.01),且中药筋脉通的疗效优于牛磺酸(P<0.01)。结论中药筋脉通对抑制糖尿病大鼠背根神经节感觉神经元及其线粒体超微结构的损伤而对糖尿病所致周围神经病变起到一定的保护作用。Objective To study the effects of Jinmaitong on ultrastructure of dorsal root ganglion（ DRG） neurons in STZ-induced diabetic rats. Methods The STZ-induced diabetic rats were randomly divided into 4 groups including normal control group（ Con）,Diabetic group（ DM）,JMT group（ JMT） and Taurine group（ Tau）. Six rats were in each group. All rats were given intragastric administration for 16 weeks and then culled. The mechanical withdrawal threshold was tested by electronic Von Frey instrument before death. The DRG of the rats was collected and used for transmission electron microscopy. Results Compared with the control group,the mechanical pain threshold of the DM group was significantly decreased（ P〈0. 01）. The ultrastructure of mitochondria in DRG neurons of the DM group was severely damaged. The number of mitochondria in neurons was significantly decreased（ P〈0. 01）,and the rate of swelling mitochondria and mitochondria vacuolar degeneration in DRG neurons was significantly increased ofthe DM group（ P〈0. 01）. The mechanical pain threshold and the number of mitochondria of two treatment groups were significantly improved compared with the DM group. The rate of swelling mitochondria and mitochondria vacuolar degeneration of two treatment groups were decreased strikingly（ P〈0. 01）,and the JMT group was more effective than the Tau group（ P〈0. 01）. Conclusion JMT could ameliorate the peripheral neuropathy by improving the pathological morphology of mitochondria in DRG neurons.

关 键 词：糖尿病周围神经病变 背根神经节神经元 筋脉通 超微结构 线粒体 

分 类 号：R285.5[医药卫生—中药学]