苦瓜总皂苷对2型糖尿病肾病大鼠肾脏组织中HIF-1α和VEGF表达的作用研究  

Effects of Momordica total saponins to HIF-1α and VEGF expression in kidney tissue of type 2 diabetic nephropathy rats

作　　者：陈伟[1] 田鲁[1] 李易[1] 王港[1] 高珊[2] 

机构地区：[1]湖北省黄石市中心医院肾内科,435000 [2]黄石市第二医院肾内科

出　　处：《环球中医药》2016年第6期659-663,共5页Global Traditional Chinese Medicine

基　　金：湖北省自然科学基金(2012FFA016)

摘　　要：目的评价苦瓜总皂苷治疗2型糖尿病肾病大鼠的疗效。方法雄性SD大鼠随机分为正常组、2型糖尿病肾病组(模型组)以及苦瓜总皂苷组(治疗组),每组16只,比较治疗4周后、8周后HIF-1α和VEGF表达特点。结果与模型组相比,治疗组尿蛋白和血清肌酐明显减少,血糖明显升高(P<0.05);HE染色可见苦瓜总皂苷对糖尿病肾病带来的肾小球基底膜增厚、系膜细胞增生有一定改善;RT-PCR和Western Blot可见:与模型组相比,治疗组大鼠肾脏组织HIF-1α、VEGF蛋白和mRNA表达明显降低(P<0.05)。结论苦瓜总皂苷可抑制HIF-1α及VEGF的表达,改善糖尿病大鼠肾功能及肾脏损害,进而延缓2型糖尿病肾病的发生发展。Objective To investigate the efficacy in type 2 diabetic nephropathy rats after treated with Mcomordica total saponins. Methods Male SD rats were randomly divided into control group,diabetic nephropathy model group and Momordica total saponins treatment group. After treatment for 4 and 8weeks,HIF-1α and VEGF expressions were detected respectively. Results Compared with the model group,there were significantly decreased in urine protein and serum creatinine,and increased in blood glucose in the treatment group（ P〈0. 05）. The pathological tissue after HE staining showed that Momordica total saponins could relieve incrassation of glomerular basement membrane,and mesangial cell proliferation. RT-PCR and Wester Blot results showed that HIF-1α and VEGF level in the treatment group were significantly lower than that in the model group（ P〈0. 05）. Conclusion Momordica total saponins could inhibit the expression of HIF-1 and VEGF,improve renal function,mitigate renal damage in diabetic rats,and delay the development of diabetic nephropathy.

关 键 词：苦瓜总皂苷 缺氧诱导因子-1Α 血管内皮生长因子 2型糖尿病 肾功能 

分 类 号：R692.39[医药卫生—泌尿科学]