葫芦科蔬菜单倍体育种技术研究进展——花粉辐射和离体胚挽救技术  

Advances of haploid breeding techniques of cucurbit vegetables: irradiated pollen and in vitro rescue

作　　者：黄金华[1] 董彦琪[1] 刘喜存[1] 王文英[1] 王玲艳[1] 朱红彩[1] 

机构地区：[1]河南省新乡市农业科学院,河南新乡453000

出　　处：《中国瓜菜》2016年第6期1-4,共4页China Cucurbits And Vegetables

基　　金：河南省科技开放合作项目(项目编号:142106000022)

摘　　要：花粉辐射和离体胚挽救技术是葫芦科作物获得单倍体胚和单倍体植株的一种重要方式。该技术在葫芦科作物育种上应用广泛,本文综述了影响该技术的一些重要因素,如基因型、辐射剂量、辐射剂量率、植株生长环境、花粉发育阶段、离体胚挽救时间、胚发育时期、染色体加倍、倍性鉴定和植株再生技术等,为该技术的开展和应用提供可参考的理论依据。The technique of parthenogenetic haploid embryos and plants through irradiated pollen and in vitro rescue is an important tool in cucurbit vegetables breeding. The technology of combining irradiated pollen pollination with embryo rescue were applied widely, many factors of this technology, such as genotype, the effects of irradiation doses,radiation dose rate,the environment of plant growth, the development stage of buds, embryo development stage and fruit rescue period,chromosome doubling and ploidy identification, and plant regeneration were reviewed.

关 键 词：葫芦科蔬菜 原位雌核发育 辐射花粉 胚挽救 染色体加倍 倍性鉴定 植株再生 

分 类 号：S63[农业科学—蔬菜学]