Developmental changes in intercellular junctions and Kv channels in the intestine of piglets during the suckling and post-weaning periods  

作　　者：Jing Wang Liming Zeng Bie Tan Guangran Li Bo Huang Xia Xiong Fengna Li Xiangfeng Kong Gang Liu Yulong Yin 

机构地区：[1]Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production [2]Key Laboratory of Agro-ecological Processes in Subtropical Region,institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125,China [3]University of the ChineseAcademy of Sciences,Beijing 10008,China. [4]Science College of Jiangxi Agricultural University,Nanchang,Jiangxi 330045,China. [5]Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients,Hunan Collaborative Innovation Center of Animal Production Safety,Changsha,Hunan 410000,China.

出　　处：《Journal of Animal Science and Biotechnology》2016年第2期228-237,共10页畜牧与生物技术杂志（英文版）

基　　金：funded by the National Key Basic Research Program of China(2013CB127302);National Natural Science Foundation of China(31330075,31372326,31301988,31301989);the State Key Laboratory of Animal Nutrition(2004DA125184F1401);the Spark Program of Jiangxi Province(20142BBF061051);Changsha Lvye Biotechnology Limited Company Academician Expert Workstation;Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine;Guangdong Hinapharm Group Academician Workstation for Biological Feed and Feed Additives and Animal Intestinal Health

摘　　要：Background： The intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different intercellular junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, intercellular junctions and voltage-gated potassium（Kv） channels in the intestine of piglets during the suckling and post-weaning periods.Results： Samples of the small intestine were obtained from 1-, 7-, 14-, and 21-d-old suckling piglets and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age. The results showed that the percentage of proliferating cell nuclear antigen（PCNA）-positive cells and alkaline phosphatase（AKP） activity, as well as the abundances of E-cadherin,occludin, and Kv1.5 m RNA and claudin-1, claudin-3, and occludin protein in the jejunum were increased from d 1to d 21 during the suckling period（P 〈 0.05）. Weaning induced decreases in the percentage of PCNA-positive cells,AKP activity and the abundances of E-cadherin, occludin and zonula occludens（ZO）-1 m RNA or protein in the jejunum on d 1, 3 and 5 post-weaning（P 〈 0.05）. There were lower abundances of E-cadherin, occludin and ZO-1m RNA as well as claudin-1, claudin-3 and ZO-1 protein in the jejunum of weanling piglets than in 21-d-old suckling piglets（P 〈 0.05）. The abundances of E-cadherin, occludin, ZO-1 and integrin m RNA were positively related to the percentage of PCNA-positive cells.Conclusion： Weaning at 14 d of age induced damage to the intestinal morphology and barrier. While there was an adaptive restoration on d 7 post-weaning, the measured values did not return to the pre-weaning levels, which reflected the impairment of intercellular junctions and Kv channels.Background： The intestinal epithelium is an important barrier that depends on a complex mixture of proteins and these proteins comprise different intercellular junctions. The purpose of this study was to investigate the postnatal and developmental changes in morphology, intercellular junctions and voltage-gated potassium（Kv） channels in the intestine of piglets during the suckling and post-weaning periods.Results： Samples of the small intestine were obtained from 1-, 7-, 14-, and 21-d-old suckling piglets and piglets on d 1, 3, 5, and 7 after weaning at 14 d of age. The results showed that the percentage of proliferating cell nuclear antigen（PCNA）-positive cells and alkaline phosphatase（AKP） activity, as well as the abundances of E-cadherin,occludin, and Kv1.5 m RNA and claudin-1, claudin-3, and occludin protein in the jejunum were increased from d 1to d 21 during the suckling period（P 〈 0.05）. Weaning induced decreases in the percentage of PCNA-positive cells,AKP activity and the abundances of E-cadherin, occludin and zonula occludens（ZO）-1 m RNA or protein in the jejunum on d 1, 3 and 5 post-weaning（P 〈 0.05）. There were lower abundances of E-cadherin, occludin and ZO-1m RNA as well as claudin-1, claudin-3 and ZO-1 protein in the jejunum of weanling piglets than in 21-d-old suckling piglets（P 〈 0.05）. The abundances of E-cadherin, occludin, ZO-1 and integrin m RNA were positively related to the percentage of PCNA-positive cells.Conclusion： Weaning at 14 d of age induced damage to the intestinal morphology and barrier. While there was an adaptive restoration on d 7 post-weaning, the measured values did not return to the pre-weaning levels, which reflected the impairment of intercellular junctions and Kv channels.

关 键 词：Intercellular junctions Kv channels Small intestine Piglets Weaning 

分 类 号：S828[农业科学—畜牧学]