ICU耐亚胺培南铜绿假单胞菌的耐药性与同源性分析  

Antibiotic resistance and genetic homology analysis of imipenem-resistant Pseudomonas aeruginosain ICU

作　　者：王玉飞[1] 张薇[1] 梁慧[1] 胡红焱[1] 刘佳[1] 杨晓莉[1] 

机构地区：[1]武警总医院检验科,北京100039

出　　处：《中华医院感染学杂志》2016年第11期2437-2440,共4页Chinese Journal of Nosocomiology

基　　金：北京市科技新星基金资助项目(Z131102000413062)

摘　　要：目的了解重症监护病房(ICU)耐亚胺培南铜绿假单胞菌感染的分子流行病学特征,为有效控制耐药铜绿假单胞菌感染及流行提供依据。方法选取2013年7月医院ICU临床送检痰液标本中分离的16株耐亚胺培南铜绿假单胞菌,采用琼脂扩散法(K-B)进行耐药性检测,脉冲场凝胶电泳(PFGE)分析菌株的同源性。结果 16株耐亚胺培南铜假单胞菌可分为5个PFGE型(A-E),其中A型为优势克隆株,占68.75%;耐亚胺培南铜绿假单胞菌对12种抗菌药物产生不同程度的耐药性,其中对多黏菌素B和氨曲南的耐药率较低,分别为0和37.5%。结论 A型克隆株是ICU耐亚胺培南铜绿假单胞菌医院感染的流行株,应加强对耐药铜绿假单胞菌的监测,防止其进一步扩散。OBJECTIVE To investigate the molecular epidemiology of imipenem-resistant Pseudomonas aeruginosaisolated from ICU patients and to provide evidence for effective control of the infection and prevalence of P.aeruginosa.METHODS Antimicrobial susceptibility testing were determined by Kindy-Bauer（K-B）disk diffusion method for the 16 strains of imipenem-resistant P.aeruginosaisolated from ICU patients in Jul.2013,and pulsed field gel electrophoresis（PFGE）was performed to analyze their genetic relationships.RESULTS The 16imipenem-resistant P.aeruginosaisolates could be divided into 5different PFGE types（A-E）and type A was the prevalent clone type（68.75%）.Imipenem-resistant P.aeruginosa strains showed different susceptibility to 12 antibiotics and drug resistance rates towards polymyxin B and aztreonam were lower,which were 0and 37.5%respectively.CONCLUSIONType A clone strains were prevalent among the imipenem-resistant P.aeruginosaisolates of PFGE in ICU infection.We should enhance the surveillance of drug-resistant P.aeruginosaand avoid its further dissemination.

关 键 词：铜绿假单胞菌 亚胺培南 耐药性 脉冲场凝胶电泳 分子分型 

分 类 号：R378.991[医药卫生—病原生物学]