拟南芥IQM5.2的克隆、表达及其生物信息学分析  

Cloning,Expression and Bioinformatics Analysis of IQM5.2 from Arabidopsis

作　　者：弓路平 萧文慧 周玉萍[1] 黄小玲[1] 田长恩[1] 

机构地区：[1]广州大学生命科学学院植物抗逆基因功能研究广州市重点实验室,广州510006

出　　处：《生物技术通报》2016年第5期69-74,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31170204;31470014);羊城学者科研项目(12A001G)

摘　　要：为了探究拟南芥IQM5基因是否存在不同的剪切方式及其器官特异性表达,利用RT-PCR技术和T-A克隆发现了IQM5基因的一种新的剪切方式,即IQM5.2。利用生物信息学方法、半定量和定量RT-PCR分析方法分别对新的CDS序列所编码蛋白的性质以及IQM5.1和IQM5.2在不同器官中的比例进行了分析。结果表明,IQM5.2编码1个由300个氨基酸组成的多肽,其N端与豌豆重金属诱导蛋白6A(HMIP6A)具有较高同源性,含有1个IQ基序,属于钙不依赖性钙调素结合蛋白;在幼苗、莲座叶、花序叶、茎和花蕾中都存在IQM5.1和IQM5.2的转录本,且二者的比例在不同的材料中不尽相同。因此,IQM5基因存在不同的剪切方式和器官特异性表达。The Arabidopsis IQM5,the fifth member of IQM family,is encoded by At5g57010. So far,there is only one splicing mode found for the IQM5 gene in the biological literatures and databases,i.e.,At5g57010.1（IQM5.1）. In this study,a new splicing mode for IQM5 gene was found by RT-PCR and T-A cloning method,designated as At5g57010.2（IQM5.2）. Bioinformatics methods and semiquantitative and quantitative RT-PCR were employed to analyze the properties of proteins encoded by new CDS sequence,and the ratio of IQM5.1 and IQM5.2 in different organs,respectively. The results revealed that IQM5.2 encoded a polypeptide of 300 amino-acid residues,and its N-terminus shared sequence homology with the pea heavy metal-induced protein 6A（HMIP6A）. The protein retained an IQ motif and was a calmodulin-binding protein of the calcium independent. There were transcripts of IQM5.1 and IQM5.2 in the seedling,rosette leaf,cauline leaf,stem,and flower bud,moreover,the ratios of them in the different organs varied.

关 键 词：IQM5.2 选择性剪切 钙调素结合蛋白 基因表达 拟南芥 

分 类 号：Q943.2[生物学—植物学]