PCR-CTPP在DNA碱基切除修复基因SNP分型中的应用  

The role of polymerase chain reaction with confronting two-pair primers in SNP genotyping of DNA base excision repair genes

作　　者：彭杨[1] 程燚[1] 杨宇馨[1] 李崇义[1] 李梦侠[1] 张诗珩[1] 王东[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肿瘤中心,重庆400042

出　　处：《重庆医学》2016年第16期2226-2228,共3页Chongqing medicine

摘　　要：目的分析两对引物-聚合酶链式反应(PCR-CTPP)在碱基切除修复(BER)途径基因单核苷酸多态性(SNP)分型中的应用,为发现新的SNP检测方法提供实验依据。方法采用PCR-CTPP扩增BER途径关键蛋白OGG1、XRCC1及APE1 4个常见SNP位点OGG1Ser326Cys、XRCC1Arg399Gln、APE1Asp148Glu及-141T/G(位于启动子区域)的DNA片段,凝胶电泳显像后判读基因型,同时与DNA测序的方式比对各基因位点的准确性。结果 PCR-CTPP方法基因分型结果与DNA测序得到的基因分型结果完全一致。结论 PCR-CTPP技术可靠、快速,其广泛应用能够有助于基因SNP的分型研究。Objective It is important to precisely determinate the single nucleotide polymorphisms （SNPs） in many genes in cluding genes related with base excision repair （BER） pathway. This research is conducted to evaluate the role of polymerase chain reaction with confronting two-pair primers （PCR-CTPP） in analyzing the SNPs of BER pathway. Methods Four common SNPs of BER pathway （OGG1 Ser326Cys,XRCC1 Arg399Gln,APE1 Asp148Glu and -141T/G in the promoter region） was detected with PCR-CTPP. 10 of the products were sent for genotype sequencing. Compare the results of PCR and sequencing to evaluate the accu racy of PCR-CTPP. Results The genotypes were exactly the same as the sequencing. Conclusion The PCR-CTPP was a reliable and rapid detective technology for SNPs genotyping. Its broadest application would be great help for gene variant analysis.

关 键 词：多态性 单核苷酸 两对引物-聚合酶链式反应 碱基切除修复途径 

分 类 号：Q78[生物学—分子生物学]