利用90K基因芯片进行小麦旗叶相关性状的QTL定位  

QTL Mapping of Flag Leaf Traits Using an Integrated High-density 90K Genotyping Chip

作　　者：连俊方[1] 张德强[1] 武炳瑾 宋晓朋[1] 马文洁[1] 周丽敏[1] 冯毅[1] 孙道杰[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100

出　　处：《麦类作物学报》2016年第6期689-698,共10页Journal of Triticeae Crops

基　　金：国家重点基础研究计划项目(2014CB138100);陕西省自然科学基金项目(2015JM3094);陕西省重点科技创新团队项目(2014KCT-25)

摘　　要：为了发掘影响小麦旗叶相关性状的QTL，以小麦骨干亲本周8425B与优良品种小偃81构建的包含102个家系的重组自交系（Recombinant inbred line,RIL）为材料，采用小麦90K SNP基因芯片技术和SSR标记对其进行分子标记检测，构建含有全基因组SNP和SSR 标记的高密度遗传图谱，并在4个环境下对小麦旗叶相关性状QTL进行检测。结果表明，所构建图谱含有6 949对多态性标记，其中，SNP标记6 910对，SSR标记39对，覆盖染色体总长度4 839.9 cM，标记间平均距离0.7 cM；A、B和D染色体组分别有2 085、4 677和187对标记，分别占总标记数的30.0%、67.5%和2.7%，标记间平均距离分别为1.0、0.6和0.8 cM。采用完备复合区间作图法共检测到22个旗叶性状加性效应 QTL，10个旗叶长 QTL 分布于 2A、3B、4B、5A、6B和7B染色体上，解释表型变异 7.900 3%-24.097 9%，除 [QX（Y15] Qfll2A1[QX）][KG-＊3]能在2个环境中检测到外，其余均为单环境QTL；4个旗叶宽 QTL分布于2A、3A和5B染色体上，解释表型变异9.079 8%-16.539 6%，其中， [QX（Y15] Qflw2A1[QX）][KG-＊3]在3个环境中均能检测到，解释表型变异12.482 6%-16.539 6%，为1个稳定的主效QTL；8个旗叶面积 QTL 分布于2A、3B、4B、5A、6B和7A染色体上，解释表型变异9.309 5%-30.498 1%，其中，3个QTL位于5A染色体上。此外，鉴定出3个分布于2A、5A和6B染色体上的 QTL富集区段。In order to carry out the quantitative trait loci（QTLs） of wheat flag leaf traits,in this study, QTLs for flag leaf traits were mapped using an available high-density 90K wheat SNP and SSR genetic linkage map developed from a recombinant inbred line（RIL） population of Zhou 8425B × Xiaoyan 81. A genetic map covering 21 wheat chromosomes was constructed,which contains 6 949 polymorphism markers（6 910 SNP and 36 SSR）,with a total genetic distance of 4 839.9 cM and an average interval distance of 0.7 cM. A,B and D chromosomes posseed 2 085,4 677 and 187 polymorphism markers,accounting for the proportion of 30.0%, 67.3% and 2.7%, respectively. And the average interval distance of the markers were 1.0,0.6 and 0.8 cM, respectively. Using phenotypic data at two locations （Yangling and Anyang） in two years（2013-2014 and 2014-2013 growing seasons）, 22 QTLs for flag leaf length（FLL）,flag leaf width（FLW） and flag leaf area（FLA） were detected by inclusive composite interval mapping（ICIM） （LOD ≥ 2. 5）. Among those QTLs, ten QTLs for FLL were mapped chromosome 2A,3B,4B,5A,6B and 7B with phenotypic variations ranging from 7. 900% to 24. 098 A stable and major QTL associated with FLL, Qfll2A-1 ,was detected in two environments,while other nine QTLs can be detected in only one environment. Four QTLs for FLW were found on chromosome 2A, 3A and 5B with phenotypic variations from 9. 080% to 16. 540%. A stable and major QTL associated with FLW, QflwgA-1 ,was detected in three environments with phenotypic variations ranging from 12. 483% to 16. 540%. Eight QTLs for FLA were mapped on chromosome 2A, 3B,4B, 5A,6B and 7A with phenotypic variations ranging from 9. 310% to 30. 498% and three of them were anchored on 5A chromosome. Three QTL enrichment sections of 2A,SA and 6B chromosomes were i- dentified.

关 键 词：小麦 重组自交系 旗叶性状 SNP QTL 

分 类 号：S512.1[农业科学—作物学] S330