湿地松侧芽增殖快繁技术研究  

Study on Propagation Technology of Lateral Buds Proliferation of Pinus elliottii

作　　者：程方[1,2] 叶建仁[1] 

机构地区：[1]南京林业大学林学院,南方现代林业协同创新中心,江苏南京210037 [2]广东省惠州市惠城区生态公益林管理站

出　　处：《现代农业科技》2016年第9期160-161,165,共3页Modern Agricultural Science and Technology

基　　金：国家林业局“948”项目“抗病松树组织培养规模化繁殖技术研究”(2011-4-69);江苏高校优势学科资助项目

摘　　要：初步探索了以湿地松幼树新生芽为外植体的侧芽增殖植株再生体系的条件,结果表明,4月中下旬为湿地松侧芽增殖最佳采样时间;20 mg/L抗坏血酸(VC)对湿地松新抽芽的褐化有一定抑制作用;6-BA的浓度为7 mg/L时,湿地松外植体的萌动最明显;改良GD+4mg/L 6-BA+0.2 mg/L NAA+1 mg/L GA3对经高浓度6-BA处理后萌动的外植体的侧芽增殖起促进作用;0、1、3、5 mg/L的GA3培养基均对新采集的外植体侧芽的诱导没有促进作用;改良GD+6 mg/L 6-BA+0.4 mg/L NAA、改良GD+8 mg/L 6-BA+0.3 mg/L NAA成功诱导出侧芽或不定芽,侧芽在后期培养中生长良好。Study on lateral buds proliferation of Pinus elliottii was done. The main resuhs were as followed ： the best acquisition time of explants was in mid-April;20 mg/L of Vc could inhibite the explants to brown;the explants germinated obviously on the media with 7 mg/L 6-BA;the explants on modified GD media with 4 mg/L 6-BA and 0.2 mg/L NAA and 1 mg/L GA3 induced lateral bud on high concentration 6-BA ; 0,1,3,5 mg/L of GA3 medium had no promating effect for the new collection of explant of lateral bud ;the explants on modified GD media with 6 mg/L 6-BA and 0.4 mg/L NAA, modified GD media with 8 mg/L 6-BA and 0.3 mg/L NAA induced lateral bud successfully, and the buds grew well in the latter time.

关 键 词：湿地松 侧芽增殖 外植体 快繁技术 新抽芽 

分 类 号：S791.246[农业科学—林木遗传育种]