变应性鼻炎大鼠动物模型的建立及评价  

The Establishment and Evaluation of Rat Model with Allergic Rhinitis

作　　者：李佳锋[1] 徐佳俊[1] 高翔[1] 徐峰[1] 钱雯靖 吴丽媛[1] 郑鸣[2] 王伟伟[1] 

机构地区：[1]湖州师范学院医学院,湖州313000 [2]福建医科大学解剖学教研室,福州350108

出　　处：《四川解剖学杂志》2016年第1期4-6,11,共4页Sichuan Journal of Anatomy

基　　金：2015年度浙江省大学生新苗人才计划(No.2015R427024);浙江省教育厅一般项目(No.Y201533594);湖州市自然科学基金项目(No.2015YZ02)

摘　　要：目的建立和评价SD大鼠变应性鼻炎模型。方法将SD大鼠随机分为对照组和模型组,模型组给予卵白蛋白(OVA)配合佐剂氢氧化铝全身致敏和局部致敏,建立SD大鼠变应性鼻炎模型;对照组以生理盐水代替OVA;HE染色检测鼻粘膜组织形态学变化;Wright’s染色检测模型组鼻腔分泌物和鼻粘膜组织嗜酸性粒细胞的表达;酶联免疫吸附测定法(ELISA)检测外周血IgE水平;通过行为学总积分对动物模型进行评价。结果模型组鼻粘膜组织纤毛脱落、倒伏,粘膜下腺体数量明显增加;模型组鼻腔分泌物中检测到大量嗜酸性粒细胞;模型组外周血IgE水平较对照组明显增高,具有统计学意义(P<0.05);滴鼻后模型组喷嚏、抓鼻的次数及流涕等行为学总积分较对照组组明显增高,具有统计学意义(P<0.05)。结论通过卵清蛋白配合佐剂氢氧化铝基础致敏和强化致敏,可成功建立SD大鼠变应性鼻炎动物模型,具有操作简单、重复性好、成功率高的优点,为今后变应性鼻炎的病变研究提供了有效的方法及组织形态学依据。Objective In this paper,the rat model with allergic rhinitis（AR）was established and evaluated.Methods The rats were divided into two groups including control group and model group.The model group with allergic rhinitis was established by the general and local sensitization with ovalbumin（OVA）,and control group were performed with normal saline instead of OVA.The morphology of the nasal mucosa was detected by HE staining,eosinophils in the nasal secretions and the nasal mucosa of model group were detected by Wright＇s staining.The level of IgE in peripheral blood was detected by enzyme-linked immunosorbent assay,and the animal model with AR was evaluated by behavioral scores.Results The model of SD rat with AR was established by the general and local sensitization with ovalbumin（OVA）.Compared with control group,the cilias on nasal mucosa tissue of model group was off or flattened,and the number of vessels,glands and eosinophile granulocytes increased,the level of IgE in peripheral blood was elevated.The behavioral scores increased in model group.Conclusion The model with allergic rhinitis can be successfully established by general and local sensitization with OVA,and possesses the advantages of simple operation and high success rate,which can provide effective methods and tissue morphology basis for the future study of allergic rhinitis.

关 键 词：变应性鼻炎 动物模型 致敏 评价 

分 类 号：R765.21[医药卫生—耳鼻咽喉科] R-332[医药卫生—临床医学]