补料培养对基因重组糖多孢红酶菌生长的影响  

Effect of 3 Levels of Feeding on Growth of Recombinant Polysaccharide

作　　者：周青阳[1] 孙艳[2] 刘宏波[1] 王立强[1] 王洪军[1] 

机构地区：[1]沈阳军区疾病预防控制中心,辽宁沈阳110034 [2]沈阳药科大学,辽宁沈阳110016

出　　处：《解放军药学学报》2016年第2期132-133,158,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：辽宁省教育厅青年人才基金项目;No.05L433

摘　　要：目的为了建立基因重组糖多孢红酶菌的生长环境平台,对培养基和生长条件进行优化。方法利用发酵罐模拟工业生产过程,采用补料3级培养的方法,对培养基成分进行筛选试验。结果培养温度为30~34℃,p H≤7.1,搅拌转速变换在700~900 r·min^-1;空气流量在0.35~0.85 vvm,最佳生长培养基：糊精30 g·L^-1、黄豆粉32 g·L^-1、ARGO玉米粉32 g·L^-1、玉米浆18 ml·L^-1、纯豆油6 ml·L^-1。结论基因重组糖多孢红酶菌在上述培养基条件下生长168 h可达到高峰。Objective To establish a platform for the growth environment of recombinant polysaccharide,and to optimize the culture medium and growth conditions. Methods The industrial production process was simulated using the method of 3 levels of feeding,and the screening test was carried out on the medium composition. Results The culture temperature ranged 30- 34 ℃,p H was ≤7. 1,the stirring speed was 700- 900 r·min^-1and air flow was 0. 35- 0. 85 vvm. The best training base was dextrin 30 g·L^-1,soybean meal 32 g·L^-1,Argo corn flour 32g·L^-1,corn steep liquor 18 ml·L^-1,pure soybean oil 6 ml·L^-1. Conclusion The growth of gene recombinant polysaccharide can reach the penk at 168 h in the above medium.

关 键 词：基因重组糖多孢红酶菌 培养基 生长优化 

分 类 号：S476[农业科学—农业昆虫与害虫防治]