HPLC法同时测定八正颗粒中栀子苷大黄素和大黄酚的含量  

Determination of Gardenoside,Emodin and Chrysophanol in Bazheng Granules by HPLC

作　　者：黎春彤[1] 史亚柱[2] 刘皈阳[1] 马建丽[1] 冯春景[1] 

机构地区：[1]解放军总医院第一附属医院药剂药理科,北京100048 [2]解放军203医院,黑龙江齐齐哈尔161000

出　　处：《解放军药学学报》2016年第2期138-140,共3页Pharmaceutical Journal of Chinese People's Liberation Army

基　　金：军队医疗机构制剂标准提高科研专项课题;No.14ZJZ20-3

摘　　要：目的建立HPLC法同时测定八正颗粒中栀子苷、大黄素和大黄酚的含量。方法色谱柱：Agilent Eclipse XDB-C18（4.6 mm×250 mm,5μm）;柱温：25℃;流动相：甲醇-0.2%磷酸溶液,梯度洗脱;流速：1.0 ml·min^-1;检测波长：254nm;检测时间：30 min。结果栀子苷、大黄素和大黄酚的线性范围为分别为10.6~212、0.515~10.3、1.01~20.2μg·mL^-1。平均回收率分别为100.7%、101.4%、100.2%,RSD分别为0.82%、0.52%、1.29%（n=6）。结论本文方法操作简便、准确度高,能缩短八正颗粒样品的检测时长,提高栀子苷、大黄素和大黄酚含量测定结果的准确性。Objective To establish an HPLC method for determining the content of gardenoside,emodin and chrysophanol in Bazheng granules. Methods Agilent Eclipse XDB-C18（ 4. 6 mm × 250 mm,5 μm） chromatographic column was used. The column temperature was 25 ℃. The mobile phase was methanol-0. 2% phosphoric acid with gradient elution. The flow rate was 1. 0 ml·min^-1and the detection wavelength was set at 254 nm. Results The linear range of gardenoside,emodin and chrysophanol was 10. 6- 212,0. 515- 10. 3 and 1. 01- 20. 2μg·mL^-1respectively. The average recovery was 100. 7%,101. 4% and 100. 2% respectively,and RSD was0. 82%,0. 52% and 1. 29%（ n = 6） respectively. Conclusion This method is simple,accurate and reliable,which may shorten the detection time of Bazheng granules and improve the determination accuracy of gardenoside,emodin and chrysophanol.

关 键 词：HPLC 八正颗粒 栀子苷 大黄素 大黄酚 含量测定 

分 类 号：R927.2[医药卫生—药学]