硫酸R15对肝素的拮抗作用  

Ability of Heparin to Reverse A Synthetic Peptide R15 Sulfate

作　　者：李通[1,2] 朱晓霞[2] 甘慧[2] 顾若兰[2] 吴卓娜[2] 孟志云[2] 窦桂芳[1,2] 

机构地区：[1]广西医科大学研究生学院,广西南宁530021 [2]军事医学科学院野战输血研究所,北京100850

出　　处：《解放军药学学报》2016年第2期148-150,共3页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的研究由15个精氨酸组成的小肽硫酸R15对未分级肝素的拮抗作用。方法通过抗凝血因子Ⅱ试验对比硫酸鱼精蛋白和硫酸R15体外中和未分级肝素的能力;在肝素化的Wistar大鼠中评价硫酸R15体内中和情况。结果抗凝血因子Ⅱ试验结果表明,体外试验中硫酸R15有更强的肝素中和能力;在体内试验中硫酸R15和硫酸鱼精蛋白中和未分级肝素的能力相似,无统计学差异。结论硫酸R15与硫酸鱼精蛋白相比有良好的药效,是一种潜在的新型肝素拮抗剂。Objective To detect the ability of heparin to reverse a synthetic peptide R15 sulfate（ RRRRRRRRRR-RRRRR-sulfate,R15S）. Methods UFH neutralization ability was compared,between protamine sulfate（ PS） and 15 S,aided by anti-FIIa assays in vitro and in heparinized rats in vivo. Results The results of anti-FIIa assaysshowed that R15 S performed better than PS in vitro,but there was no statistical difference between PS and R15 S in UFH neutralization ability in vivo. Conclusion R15 S possesses good efficacy for UFH reversal,which can be developedas a potential UFH reversal agent.

关 键 词：硫酸鱼精蛋白 未分级肝素 过敏反应 

分 类 号：R965[医药卫生—药理学]