HPLC法测定黄药子散中黄芩苷含量  

Determination of Baicalin in Huangyaozi Pulvis by HPLC

作　　者：王杰松[1] 史宁[1] 张华[1] 钱奇芳 张毓敏[1] 常李荣[1] 

机构地区：[1]解放军306医院药学部,北京100101

出　　处：《解放军药学学报》2016年第2期166-167,共2页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的建立黄药子散中黄芩苷含量测定的HPLC方法。方法色谱柱为NUCLEODUR C18Pyramid（250 mm×4.6mm,5μm）,流动相为乙腈-水（26∶74,含0.5%三乙胺,磷酸调p H 3.0）,流速为1 ml·min^-1,检测波长为278 nm,进样量为10μl,柱温为30℃。结果回归方程为Y=31.70 X-23.00,r=0.9999,黄芩苷的浓度线性范围是7.72~193.0μg·mL^-1。平均加样回收率为101.4%,RSD为1.4%（n=6）。结论该方法操作简便、稳定性好,可用于黄药子散中黄芩苷的含量测定。Objective To establish a quantitative method for the determination of baicalin in Huangyaozi pulvis by HPLC. Methods The chromatographic conditions involved NUCLEODUR C18Pyramid（ 250 mm × 4. 6 mm,5 μm）. The mobile phase was acetonitrile and water（ 26∶ 74,containing 0. 5% triethylamine,and p H was adjusted to 3. 0 by phosphoric acid）. The flow rate was 1 ml·min^- 1,sample size 10 μl,and column temperature 30 ℃ and monitored at 278 nm. Results The regression equation was Y = 31. 70 X- 23. 00,r = 0. 9999 and linear range was 7. 72- 193. 0 μg·mL^-1. The average recovery of baicalin was 101. 4%,RSD = 1. 4%（ n = 6）. Conclusion This method is simple,quick,accurate and reliable,which can be used for determination of baicalin in Huangyaozi pulvis.

关 键 词：黄药子散 黄芩苷 HPLC 

分 类 号：R927.2[医药卫生—药学]