氯胺酮对大鼠海马神经元ATP酶活性的影响  

Effect of Ketamine on the activity of Atpase in Rats hippocampal Neurons

作　　者：周彤[1] 曹馨方[1] 陈希[1] 陈蕊[1] 刘沫[1] 张宇[1] 魏成威[1] 吴越[1] 于东旭[1] 刘子睿[1] 姜仁礼 李欣然[1] 王冠颖[1] 肖建华[1] 高利[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《中国兽医杂志》2016年第5期10-13,共4页Chinese Journal of Veterinary Medicine

基　　金：国家自然科学基金(31372491);黑龙江省自然科学基金(C201113);黑龙江省教育厅基金(12531016)

摘　　要：本试验研究不同浓度氯胺酮对大鼠海马神经元ATP酶活性的影响,并探讨氯胺酮的中枢麻醉机制。体外培养怀孕16-18 d的胎鼠神经细胞,至第8天直接给予3种不同浓度的氯胺酮,使用超微量ATP酶测定试剂盒,检测胎鼠海马中ATP酶的活力。结果显示,海马中Na＋-K＋-ATP酶和Ca2＋-Mg2＋-ATP酶的活性均受到不同程度的抑制,并且这种抑制作用的程度具有剂量依赖的趋势。据此推测氯胺酮的麻醉机制可能与抑制海马中Na＋-K＋-ATP酶和Ca2＋-Mg2＋-ATP酶的活性有关。In this study,we investigated the mechanisms of ketamine anesthesia on the central nervous system by examing the effect of different concentrations of ketamine on the activity of Atpase in Rats hippocampal Neurons.Fetal rat neurons from 16-18days of pregnancy fetal rats were incubated at day 15 in culture with three different concentrations of ketamine,and adenosinetriphosphatase assay kit was used to detect the activity of ATPase in the Fetal rat hippocampal.Our results showed that ketamine concentration-dependently suppressed the activity of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase. These data indicate that central anesthetic effect of ketamine may be related to the reduction in the activity of Na＋-K＋-ATPase and Ca2＋-Mg2＋-ATPase.

关 键 词：氯胺酮 麻醉 NA+-K+-ATP酶 Ca2+-Mg2+-ATP酶 

分 类 号：S857.144[农业科学—临床兽医学]