接骨方含药血清孵育体外培养骨痂中破骨细胞、成骨细胞和软骨细胞生长的影响  

The Effect of Jiegufang Drug Serum on Callus Osteoclasts,Osteoblasts and Chondrocytes in Vitro Growth

作　　者：尹拥军 王炳南[2] 林勇[2] 

机构地区：[1]南海经济开发区人民医院小塘分院,佛山528222 [2]广州中医药大学,广东510405

出　　处：《世界中医药》2016年第4期671-674,共4页World Chinese Medicine

摘　　要：目的:观察接骨方含药血清对体外培养的破骨细胞、成骨细胞和软骨细胞生长的影响。方法:取新西兰家兔骨折造模骨痂进行体外培养,制备接骨方含药血清,用接骨方含药血清孵育新西兰家兔骨痂,进行破骨细胞、成骨细胞和软骨细胞镜下形态学观察,观察骨痂中软骨细胞、成骨细胞和破骨细胞的数目。结果:术后10 d的新西兰家兔骨痂镜下可见细胞开始从组织块周围爬出;染色后可见胞浆内出现黄褐色颗粒,胞核饱满,核仁清晰;术后17 d观察组骨痂可见成骨细胞和软骨细胞的增殖明显,破骨细胞增殖相对成骨细胞和软骨细胞较慢,术后24 d、31 d时可见骨痂中破骨细胞、成骨细胞和软骨细胞均增殖速度变缓,与对照组相比,术后10 d、17 d、24 d、31 d观察组骨痂中成骨细胞和软骨细胞的增殖情况明显优于对照组;与对照组相比,术后10 d、17 d、24 d、31 d观察组骨痂中破骨细胞数量少于对照组,2组间差异无统计学意义(P>0.05);与对照组相比,术后10 d、17 d、24 d、31 d观察组骨痂中成骨细胞和软骨细胞数目均多于对照组,2组有统计学意义(P<0.05)。结论:接骨方含药血清可促进新西兰家兔成骨细胞和软骨细胞增殖,具有促进骨组织的再生的功效。Objective： To observe the effect of Jiegufang drug serum on callus osteoclasts,osteoblasts and chondrocytes in vitro growth. Methods： To culture New Zealand rabbits fracture modeling callus in vitro and prepare Jiegufang drug serum to incubate New Zealand rabbits callus. The morphological shape and the number of callus osteoclasts,osteoblasts and chondrocyte were observed. Results： After 10 d of surgery,cells began to climb out of the surrounding tissue blocks. After staining,brown granules in the cytoplasm,nucleus full and clear nucleolus could be seen. After 17 d of surgery,it can be seen that the proliferation of osteoblasts and chondrocytes grew fast and osteoclasts grew slow in the observation group. After 24 d,31 d of surgery,osteoclasts,osteoblasts and chondrocytes grew slower. To compare with the control group,the the proliferation of osteoblasts and chondrocyte were faster than the control group after 10 d,17 d,24 d,31 d of surgery. After surgery of 10 d,17 d,24 d,31 d,the number of osteoclasts in the observation group was lesser than the control group,but the difference was not statistically significant（ P〈0. 05）.Compared with the control group,the number of osteoblasts and the chondrocytes in the observation group were more than the control group after 10 d,17 d,24 d,31 d of surgery,and the difference was significant（ P〈0. 05）. Conclusion： Jiegufang drug serum can promote New Zealand rabbits osteoblasts and chondrocytes proliferation,and thus to promote the regeneration of bone tissue.

关 键 词：接骨方含药血清 新西兰家兔骨痂 破骨细胞 成骨细胞 软骨细胞 分化 

分 类 号：R681.3[医药卫生—骨科学]