益气解毒方对急性期与慢性期溃疡性结肠炎大鼠TNF-α水平的影响  

The Effect of Yiqi Jiedu Formula on the Serum Levels of TNF-α in the Acute and Chronic Phase in Rats with Ulcerative Colitis

作　　者：常孟然 林燕[1] 李文静[1] 赵程博文 

机构地区：[1]北京中医药大学基础医学院,北京100029

出　　处：《世界中医药》2016年第4期675-678,共4页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目--基于线粒体能量代谢探讨溃疡性结肠炎脾虚为本的病理基础(编号:81202682)

摘　　要：目的:探讨益气解毒方对急性期和慢性期溃疡性结肠炎(Ulcerative Colitis,UC)大鼠血清TNF-α水平的影响。方法:利用家兔结肠黏膜-TNBS/乙醇的免疫复合法造模,将100只大鼠随机分为正常组、模型组(各30只)、益气解毒组和西药组(各20只),观察急性期与慢性期的疗效并检测血清TNF-α水平。结果:造模完成后,模型组TNF-α水平明显高于正常组(P<0.01),急性期和缓解期与造模后第1天相比有所下降低(P<0.05),但仍显著高于正常组(P<0.01)。在急性期,益气解毒组与西药组的水平均低于模型组(P<0.05),高于正常组(P<0.05,P<0.01);西药组低于益气解毒组(P<0.05)。在缓解期,益气解毒组与西药组水平保持下降,与正常组有统计学意义(P<0.05),与模型组统计学意义明显(P<0.01),2用药组间的水平相当。益气解毒组的水平在缓解期显著下降,明显低于其急性期(P<0.05)。在缓解期与急性期相比下降幅度方面,益气解毒组较美沙拉嗪明显。结论:益气解毒方与美沙拉嗪整体效果相当,美沙拉嗪能够发挥良好的抗炎效果,适用于急性炎症损伤期,益气解毒方抗炎效果稍逊于美沙拉嗪,但在加快溃疡的愈合及黏膜修复增生的速度方面已达到美沙拉嗪水平,在抗炎与调节免疫功能上优于美沙拉嗪。Objective： To study the effect of Yiqi Jiedu formula on the serum levels of TNF-α during the acute and chronic phase in rats with ulcerative colitis（ UC）. Methods： Using immune and chemical methods（ rabbit colonic mucosa sensitized and TNBS /50% alcohol coloystered） to acquire UC models,then randomly divide one hundred rat models into four groups： normol group（ 30）,model group（ 30）,Yiqi Jiedu formula group（ 20） and mesalazine group（ 20）. To observe the curative effect and test the serum level of TNF-α during the acute and chronic phase of UC. Results： After acquired UC rat model,the serum level of TNF-α in model group is significantly higher than the normal group（ P〈0. 01）. Compared with the first day of modeling,the level in the acute and chronic phase is lower in the UC rat model（ P〈0. 05）,but it is significantly higher than that of the normal group（ P〈0. 01）. In the acute phase,the serum TNF-α levels of Yiqi Jiedu formula group and mesalazine group are lower than the model group（ P〈0. 05）,but is higher than that of the normal group（ P〈0. 05,P〈0. 01）. Moreover,the level of the mesalazine group is lower than that of the Yiqi Jiedu formula group（ P〈0. 05）. In the chronic phase,the serum TNF-α levels of Yiqi Jiedu formula group and mesalazine group kept falling. The difference of both groups between the normal group decreased（ P〈0. 05）,while the level are still significantly higher than the normal group（ P〈0. 01）. However,the gap between the two groups are narrow. The decrease degree of Yiqi Jiedu formula group is more obvious in acute phase than chronic phase（ P〈0. 05）. In regards to the decrease gap between the acute phase and chronic phase,the Yiqi Jiedu formula group is significantly greater than that of the mesalazine group. Conclusion： The overall effect of Yiqi Jiedu formula is similar to mesalazine. Mesalazine can play a well role on controlling inflammation,so it can be used in acute phase. While in the respect

关 键 词：溃疡性结肠炎 TNF-Α 益气解毒方 急性期 慢性期 

分 类 号：R442.2[医药卫生—诊断学]