基于主成分分析的油菜幼苗铝毒机制研究  

Study on Aluminum Poisonous Mechanism for Rape Seedling Based on Principal Component Analysis

作　　者：崔雪梅[1] 郭海如[2] 李春生[1] 简君萌 

机构地区：[1]湖北工程学院生命科学技术学院/特色果蔬质量安全控制湖北重点实验室,湖北孝感432000 [2]湖北工程学院计算机学院,湖北孝感432000

出　　处：《河南农业科学》2016年第5期52-55,共4页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(71473072);湖北省中青年人才项目(Q20152702)

摘　　要：为探究油菜幼苗对Al^(3+)的耐受性及生理响应机制,设置7个Al^(3+)浓度试液,用水培法处理生长2周的油菜幼苗,胁迫1周后测定油菜的株高、根长的变化量,根系活力,根系氧化力,以及可溶性糖、叶绿素a、叶绿素b、总叶绿素、游离脯氨酸、H_2O_2含量共10项指标,并进行主成分分析。结果表明,对照(Al^(3+)浓度为0μmol/L)各项生理指标表现最好,随着Al^(3+)浓度的增加,各项指标表现越来越差;Al^(3+)胁迫对叶绿素a、总叶绿素含量和根长的影响最大,其次是根系活力、叶绿素b含量和根系氧化力。说明铝胁迫首先降低了油菜幼苗的光能利用率,抑制根系生长,同时根系活力及氧化力下降,从而影响植株对水分及养分的吸收。To explore the tolerance and physiological response of rape seedling to Al^（3＋）,we set testing solution of seven Al^（3＋）densities,and processed two week-long rape seedlings by water treatment. After one week we measured the ten indicators： plant height,root length,root activity,root oxidizing ability,soluble sugar,chlorophyll a（ Chla）,chlorophyll b（ Chlb）,total chlorophyll（ Chlt）,proline and H_2O_2 content,and used principal components analysis. Results showed as followed： each physiological indicator was best when the density of Al^（3＋）was 0 μmol / L; the indicators got worse with the increase of Al^（3＋）concentration;the influence of Al^（3＋）on Chla,Chlt and root length was biggest,and next was the influence on root activity,Chlb and root oxidizing ability. It indicates that aluminum stress reduces the utilization of solar energy,inhibits the root growth,and reduces the root activity and root oxidizing ability,thereby affecting the plant＇s absorption of water and nutrients.

关 键 词：油菜幼苗 主成分分析 铝胁迫 根系 

分 类 号：S565.4[农业科学—作物学]