低能N^+注入选育凝结芽孢杆菌高效生防菌株  

Mutation Breeding of High-efficiency Biocontrol Strain of Bacillus coagulans by Low Energy N^+ Ion Beam Implantation

作　　者：顾鹏飞[1] 吴刚[1] 胡永红[1] 王春晓[1] 丁志雯[1] 杨文革[1] 

机构地区：[1]南京工业大学生物与制药工程学院,江苏南京211816

出　　处：《河南农业科学》2016年第5期87-90,共4页Journal of Henan Agricultural Sciences

基　　金：江苏省农业科技自主创新资金项目(CX(14)2057);江苏省科技支撑计划(农业)项目(BE2014386)

摘　　要：为了进一步提高凝结芽孢杆菌NJYHHWG 877005菌株的拮抗性能,获得生防效果更好的菌株,利用低能N^+注入菌株进行诱变,并通过平板对峙培养对诱变处理后的菌株进行筛选。结果表明,菌株存活率曲线遵循N^+注入生物效应的马鞍型曲线,根据其存活率及突变率确定N^+最佳注入能量为15 ke V,最佳注入剂量为140×10^(13)个/cm^2。通过筛选获得1株具有良好性状的突变株L1,芽孢形成率达77.42%,对灰葡萄孢霉菌的抑制率高达87.81%,分别较原始出发菌株提高了23.79、11.71个百分点,且连续传代培养8次,遗传稳定性良好。In order to improve the antagonistic ability of Bacillus coagulans NJYHHWG 877005 and search for high-efficient mutant strains,the strain was induced by low energy N~＋ion beam implantation and the mutation strains were screened by confrontation experiments. It was found that the curve of survival took a ＂saddle shape＂with N~＋ion dose increased. By the livability and mutation rate of the strain,the best induced mutation action was decided when the used energy and ions implantation dose were 15 ke V and 140 × 10^（13）N~＋/ cm^2. A fine strain L1 was obtained through screening. The forming rate of spore was up to 77. 42%,and the inhibition rate of the mutant strain against Botrytis cinerea was up to 87. 81%,increased by 23. 79 and 11. 71 percentage points compared to the primitive strain,respectively. After eight generations of propagation,the mutant strain showed high genetic stability.

关 键 词：凝结芽孢杆菌 N+注入 诱变选育 遗传稳定性 抑菌率 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治] Q319.2[农业科学—植物保护]