转抗菌肽D基因中华猕猴桃的鉴定及抗病性相关酶活性研究  

Identification of Transgenic Actinidia chinensis Plants with Antibacterial Peptide D Gene and Analysis of Disease-resistant Related Enzyme Activities

作　　者：韦鹏飞[1] 申炎龙 叶霞[1] 郑先波[1] 谭彬[1] 李继东[1] 冯建灿[1] 

机构地区：[1]河南农业大学,河南郑州450002

出　　处：《河南农业科学》2016年第5期130-134,共5页Journal of Henan Agricultural Sciences

基　　金：河南省现代农业产业技术体系大宗水果产业技术创新团队项目(S2014-11-G02);河南省高校科技创新团队支持计划项目(14IRTSTHN011)

摘　　要：以转抗菌肽D基因的中华猕猴桃抗性芽为材料,通过诱导生根和移栽获得猕猴桃盆栽苗。对盆栽苗进行PCR和RT-PCR鉴定表明,抗菌肽D基因已经整合到猕猴桃基因组中,并进行了转录。对盆栽苗叶片中的过氧化氢酶（CAT）、过氧化物酶（POD）和苯丙氨酸解氨酶（PAL）的活性分析结果表明,在喷施猕猴桃细菌性溃疡病病原菌（1.2×10~7~1.2×10~8,OD600值为0.2）的条件下,转基因植株的CAT和POD活性比未转基因植株明显降低,PAL活性比未转基因植株明显升高。In the present study,the transgenic Actinidia chinensis shoots with antibacterial peptide D gene was used as materials,and many Actinidia chinensis plants via root induction and transplanting in greenhouse were obtained. The PCR and RT-PCR analysis results of transgenic kiwifruit plants indicated that antibacterial peptide D had been integrated in the Actinidia chinensis genome and transcribed. The activity analysis of catalase（ CAT）,peroxidase（ POD）,and phenylalanine ammonialyase（ PAL） showed that in response to canker bacteria treatment,enzyme activities of CAT and POD in transgenic plants were greatly reduced,and PAL enzyme activity was enhanced compared with the controls.

关 键 词：中华猕猴桃 抗菌肽D 过氧化氢酶 过氧化物酶 苯丙氨酸解氨酶 

分 类 号：S663.4[农业科学—果树学]