知母活性成分ZMS对老年大鼠脑M受体的调节作用  

The effect of ZMS on brain M receptor in aged rats

作　　者：胡梅[1] 胡雅儿[1] 张蔚[1] 夏宗勤[1] 

机构地区：[1]上海第二医科大学细胞调控研究室,200025

出　　处：《中华核医学杂志》2001年第3期158-161,共4页Chinese Journal of Nuclear Medicine

基　　金：国家自然科学基金资助项目! ( 3 95 70 83 5 )

摘　　要：目的　探讨知母活性成分ZMS整体用药后是否对脑M2 受体有上调作用。方法　以Methoctramine为选择性非标记配基 ,3 H 二苯羟乙酸奎宁酯为非选择性标记配基 ,建立M2 亚型的放射配基竞争结合分析方法。结果　与青年大鼠相比 ,老年大鼠脑M2 受体及总M受体减少 ,ZMS同时上调脑M2 受体及总M受体 ,且与动物的学习记忆能力呈显著正相关。结论　ZMS能上调脑M2 受体 ,这很可能是知母改善脑学习记忆功能的重要因素之一。Objective The purpose of this work was to study the effect of ZMS, an active component of Yin tonic, Zhimu, on brain M 2 receptor density of aged animals and its correlation with the effect on learning/memory ability. Methods A dual site competitive binding assay using 3H quinuclidinyl benzilate (QNB) as non selective radioligand and unlabelled Methoctramine as selective competitive agent was established for measuring M 2 receptor density in aged rats. Results In addition to the change of total density of M receptors, the density of a subtype of M receptors,M 2 receptor in brain was significantly decreased in aged rats [(231.8±115.9) fmol·mg -1 ( ±s ) in young rats and (97.9±46.3) fmol·mg -1 in aged rats]. When the aged rats were treated with ZMS for two months, in addition to the up regulation of total M receptors, the M 2 receptor was up regulated significantly [being (213±77) mg at a ZMS dose of 3.6 mg·kg -1 ·d -1 , and (212±72) mg at a ZMS dose of 18 mg·kg -1 ·d -1 ]. When the correlation between M 2 or total M receptor densities and the learning/memory ability measured by Y maze performance was examined with linear regression, the correlation coefficient was remarkable (0 721 and 0.505, respectively). Conclusions ZMS has the ability of up regulating M 2 receptor and this may be an important factor for the improvement of learning and memory by ZMS.

关 键 词：知母 皂甙元 阿尔茨海默病 学习 记忆 受体 毒蕈碱 

分 类 号：R285[医药卫生—中药学]