神经营养素3在面神经的转运研究  

The study of transport of neurotrophin-3 in facial nerve

作　　者：李云春[1] 王全林[1] 杨晓川[1] 李林[1] 何刚[2] 高炳庆[2] 林代诚[2] 

机构地区：[1]华西医科大学附属第一医院核医学科,成都610041 [2]华西医科大学附属第一医院耳鼻咽喉科,成都610041

出　　处：《中华核医学杂志》2001年第3期185-186,共2页Chinese Journal of Nuclear Medicine

基　　金：高等学校博士学科点专项科研基金资助!( 960 63 0 15 )

摘　　要：目的　探讨面神经再生微环境及神经营养素 3(NT 3)对神经细胞的作用。方法　将新西兰兔一侧面神经干横切断后置入硅胶再生室 ,再将13 1I NT 3或12 5I NT 3或13 1I 人血清白蛋白 (HSA)(10 μL/只 ,约 7 4MBq)注入再生室 ,取注入13 1I NT 3或13 1I HSA的兔分别在注药后不同时刻行头部冠状位平面显像 ,取注入12 5I NT 3兔的面神经干、脑干和对侧面神经干测定其转运率。另取置入再生室的兔同时在再生室注入 1mgNT 3或 5 4mgHSA和 7 4MBq13 1I NT 3后 ,不同时刻行头部冠状位平面显像。结果　注药后 4h有 4 0 5 %的12 5I NT 3转运到面神经干中 ,12h为最高峰 (2 7 0 4% ) ;8h有9 85 %转运至脑干 ,2 4h转运至脑干的量达 41 18% ,但13 1I HSA在面神经中无转运 ,且13 1I NT 3在面神经的转运受NT 3的明显抑制。结论　NT 3在面神经中存在受体介导逆行转运。Objective To study the transport of neurotrophin 3(NT 3) in facial nerve using radioactive tracer Methods After one of the two facial nerves of an adult rabbit was transected, a silicone chamber was inserted between the two stumps, and 7 4 MBq of 125 I NT 3 was administered into the chamber At different time points after injection, ratioactivities of both facial nerves and brain stem were counted, respectively 7 4 MBq of 131 I NT 3 or 131 I HSA was administered into the chambers of one group of two rabbits. 1 mg of NT 3 or 5 4 mg of HSA and 7 4 MBq of 131 I NT 3 were administered into the chambers of the another group of two rabbits The eight rabbits were imaged at coronal section of head at different time points. Results 4 05% of 125 I NT 3 was transported into facial nerve stumps from the chamber at 4 h after injection The peak transport rate of 125 I NT 3 in facial nerve was shown at 12 h, the peak value was 27 04% 12 16% and 41 18% of 125 I NT 3 were transported into brain stem of experimental side at 12 h and 24 h,respectively But, 131 I HSA wasn't transported in facial nerve The transport of 131 I NT 3 was markedly restrained by NT 3 in facial nerve Conclusion NT 3 has a receptor mediated axonal retrograde transport in facial nerve

关 键 词：神经营养素3 面神经 碘放射性同位素 放射测量术 

分 类 号：R965[医药卫生—药理学]