妇科养坤丸中4种有效成分提取工艺的响应面优化及HPLC测定  

Extraction Optimization by Response Surface Method and Content Determination by HPLC of Four Effective Components in Fuke Yangkun Pills

作　　者：齐广才[1] 崔生飞[1] 吴江瑞 张咪咪[1] 刘珍叶[1] 

机构地区：[1]延安大学化学与化工学院,陕西延安716000

出　　处：《中国药师》2016年第6期1048-1051,1063,共5页China Pharmacist

摘　　要：目的:建立同时测定妇科养坤丸中芍药苷、甘草苷、黄芩苷和木香烃内酯含量的HPLC法并通过响应面优化法分别对4种成分的提取工艺进行优化。方法:采用Kromasil C_(18)色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈(A)-0.2%磷酸溶液(B),梯度洗脱,流速为1.0 ml·min^(-1),检测波长为230,276,280,225 nm,柱温为28℃。以4种成分含量为指标,选取甲醇浓度、料液比和提取时间为考察因素,采用响应面优选妇科养坤丸中4种有效成分的提取工艺。结果:芍药苷、甘草苷、黄芩苷和木香烃内酯分别在1.616~161.600,0.432~43.180,2.045~204.500,0.518~51.840μg·ml^(-1)呈良好的线性关系,平均回收率(n=9)为98.3%,99.6%,97.9%,98.1%。获得的最佳工艺条件为甲醇浓度64%,料液比1∶51,提取时间为25 min。结论:采用响应面优化妇科养坤丸中4种有效成分的提取工艺,方法简便,预测性良好。所建立的含量测定方法简便、准确,可用于妇科养坤丸的质量控制。Objective： To develop an HPLC method for the simultaneous determination of paeoniflorin, liquiritin, baicalin and costunolide in Fuke Yangkun pills, and optimize the extraction technology by response surface methodology （RSM）. Methods： The separation of targeted compounds were performed on a Kromasil CIs column （250 mm × 4.6 mm, 5 μm）. The mobile phase consisted of aeetonitrile （A） and 0.2% phosphoric acid （B） with gradient elution at a flow rate of 1.0 ml ·min-1. The detection wavelength was set at 230, 276,280 and 225 nm, respectively. The column temperature was 28℃. Using the contents of the four components as the indices, the extraction process was optimized by a response surface method with methanol concentration, solid-liquid ratio and ex- traction time as the influencing factors. Results： The linear range of paeoniflorin, liquiritin, baicalin and costunolide was 1. 616- 161. 600, 0. 432-43. 180, 2. 045-204.500 and 0.518-51. 840 μm · m1-1, respectively. The average recovery （ n = 9 ） was 98.3% , 99.6% , 97.9% and 98.1%, respectively. The optimum conditions of extraction process were as follows： the methanol concentration was 64%, the solid-liquid ratio was 1 ： 51, and the extraction time was 25 min. Conclusion： The response surface methodology is convenient and highly predictive in optimizing the extraction process of the four effective components in Fuke Yangkun pills. The devel- ooed content determination method is simole and accurate, which can be used for the quality control of Fuke Yangkun pills.

关 键 词：妇科养坤丸 芍药苷 甘草苷 黄芩苷 木香烃内酯 高效液相色谱法 含量测定 响应面分析 

分 类 号：R284.2[医药卫生—中药学]