一测多评法同时测定酸枣仁中4种皂苷类成分的含量  

Determination of Four Kinds of Saponins in Spina Date Seed by Quantitative Analysis of Multi-components by Single Marker

作　　者：黄丽 万佐玺[2] 

机构地区：[1]恩施自治州中心医院,湖北恩施445000 [2]湖北民族学院生科院

出　　处：《中国药师》2016年第6期1073-1076,共4页China Pharmacist

摘　　要：目的:建立一测多评(QAMS)的方法用来测定酸枣仁中4种皂苷类成分的含量。方法:以酸枣仁皂苷A为参照物,采用HPLC法,以Kromasil C_(18)色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸水溶液(30∶70);流速:1.0 ml·min^(-1);检测波长:280 nm;柱温:32℃;进样量:20μl。应用QAMS法的方法测定4种活性皂苷成分之间的相对校正因子,同时采用外标法测定该药材中4种成分的含量,得出的结果与QAMS法测定的结果进行比较。计算QAMS法测得含量计算结果(Wf)和外标法的测得含量测定结果(Ws)的相对误差,验证QAMS法的准确性。并对其重现性进行考察。结果:建立QAMS法用于测定酸枣仁中4种皂苷类成分的含量,并对16批酸枣仁药材进行测定,其计算值与测定值的差异较小(RSD<5%)。结论:该方法操作简单、专属性和重复性良好,在对照品缺乏的情况下,可以作为一个新模式用于酸枣仁药材的多成分定量。QAMS法测对酸枣仁的质量评价模式得到了验证,可用于酸枣仁药材的质量控制,并可为后续的QAMS研究提供参考价值。Objective： To develop a method of quantitative analysis of multi-components by single marker （QAMS） for 4 kinds of saponins in spina date seed. Methods： Using jujuboside A as a reference, an HPLC method with a Kromasil Cls column （250 mm x 4.6 mm, 5μm） was applied, the mobile phase was acetonitrile-0.1% phosphoric acid （30 ： 70）, the flow rate was 1.0 ml - min - 1, the detection wavelength was 280 rim, the column temperature was 32 ℃ and the injection volume was 20 I.d. The relative cor- rection factors among the four kinds of saponins were detected by QAMS. An external standard method was also used to determine the contents of the four saponins （ Ws）, and the results were compared with those of QAMS （ Ws ）, and then the relative errors were calcu- lated between Wf and W, to evaluate the accuracy of QAMS, and the reproducibility was investigated as well. Results： The established QAMS was used to determine the four kinds of saponins in spina date seed, and totally 14 batches of spina date seed were determined. No significant differences were found out between the calculated values and the determined ones （RSD 〈 5% ）. Conclusion： The vali- dated HPLC method shows the advantages of precision,reproducibility and reliability. The established QAMS method is feasible, and suitable for the quality control of saponins in spina date seed.

关 键 词：一测多评法 酸枣仁 外标法 酸枣仁皂苷A 

分 类 号：R917[医药卫生—药物分析学]