针刺对血管性痴呆大鼠海马突触传递相关信号分子的影响  

Effects of Acupuncture on Hippocampal Synaptic Transmission Signal Molecules in Rats with Vascular Dementia

作　　者：李慧[1] 王雪蕊[2] 杨静雯[2] 杜斯琪 朱雯[2] 姬彩硕 刘存志[2] 

机构地区：[1]天津中医药大学,天津300193 [2]首都医科大学附属北京中医医院针灸中心,北京100010

出　　处：《中国中医药信息杂志》2016年第7期77-81,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(2014CB543203);北京市科技新星计划(2014B063)

摘　　要：目的观察针刺对血管性痴呆(VD)大鼠海马突触传递相关信号分子蛋白激酶C(PKC)、钙调蛋白激酶(Ca MKⅡ)、N-甲基-D-天冬氨酸受体2B亚基(NR2B)的影响,探讨针刺治疗VD的分子机制。方法采用栓子注入方法制作多发性脑梗死痴呆模型,实验大鼠随机分为正常组、模型组、针刺组和非穴组。针刺组针刺"足三里"治疗,非穴组于肋下髂嵴上10 mm(非穴)处针刺治疗,正常组和模型组大鼠施以与针刺组和非穴组相同强度的捉抓刺激。治疗结束后,ELISA检测PKC活性,Western blot检测Ca MKⅡ蛋白表达;免疫组化检测CA1、CA3及DG区NR2B蛋白表达。结果 VD大鼠海马PKC活性、NR2B蛋白表达较正常组显著下降(P<0.01);针刺治疗后,PKC活性明显上升(P<0.05),NR2B蛋白表达水平有上升的趋势。各组Ca MKⅡ蛋白表达无明显变化。结论针刺增强海马神经元突触传递信号分子PKC活性,这可能是其治疗VD的重要分子靶点之一。Objective To observe the effects of acupuncture on synaptic transmission signal molecules in rats with vascular dementia (VD), such as PKC, CaMKⅡ and NR2B, and discuss the molecular mechanism of acupuncture treatment for VD.Methods The multi-infarct dementia model was established by injection of emboli into the internal carotid artery. Experimental rats were randomly divided into normal group, model group, acupuncture group and non-acupoint group. For acupuncture group, acupuncture needles were penetrated into bilateral Zusanli. Non-acupoint group was given acupuncture treatment at the bilateral hypochondrium (10 mm above iliac crest). The rats in normal group and model group were performed to the same amount of capture stimulation as the acupuncture and non-acupoint groups. After treatment, the hippocampal PKC activity was detected by ELISA. Western blot was used to detect CaMKⅡ expression, and the protein expression of NR2B in CA1, CA3 and DG zones was assayed by immunohistochemical staining.Results Compared with normal group, PKC activity and NR2B expression in the hippocampus significantly decreased in the model group (P<0.01). After the acupuncture treatment, PKC activity increased significantly (P<0.05), and the protein expression of NR2B showed a trend to increase. There was no obvious difference in CaMKⅡ expression among all groups.Conclusion Acupuncture at Zusanli can enhance the activity of hippocampal PKC, a synaptic transmission signal molecule, which maybe one of the important molecular targets for the treatment of VD.

关 键 词：针刺 血管性痴呆 海马 组织蛋白激酶C 钙蛋白激酶 N-甲基-D-天冬氨酸受体2B亚基 大鼠 

分 类 号：R245[医药卫生—针灸推拿学]