HPLC法测定结核性胸膜炎患者胸腔积液中莫西沙星的浓度  

Determination of moxifloxacin concentration in pleural effusion of tuberculous pleurisy patients by HPLC

作　　者：邓斐[1] 金家贵[1] 王蓝天[2] 陈章[3] 雍小兰[2] 

机构地区：[1]成都医学院,四川成都610500 [2]成都军区总医院临床药学科,四川成都610083 [3]成都军区总医院感染呼吸科,四川成都610083

出　　处：《中国药物应用与监测》2016年第3期140-143,共4页Chinese Journal of Drug Application and Monitoring

基　　金：四川省科技厅基金项目(42412D1Y)

摘　　要：目的:建立测定结核性胸膜炎患者胸腔积液中莫西沙星浓度的方法。方法:利用高效液相色谱方法测定胸水中莫西沙星的浓度。色谱柱:Agilent Eclipse XDB-C18柱(150 mm×4.6 mm,5μm);流动相:乙腈-水(含0.05%三氟醋酸,23︰77);流速为1.0 m L·min^(-1);紫外检测波长为296 nm(0~2.5 min),256 nm(2.5~4.5 min),296 nm(4.5~8.0 min);柱温:25℃;进样量:30μL。结果:莫西沙星血药浓度在0.1~10.0μg·m L^(-1)范围内线性关系良好(r=0.999 2),定量下限为0.1μg·m L^(-1),低、中、高3个质量浓度的日内精密度RSD分别为2.76%、2.23%和2.00%,日间精密度RSD分别为5.88%、3.49%和3.60%,三者提取回收率及内标的提取回收率分别为53.00%、63.26%、65.42%和91.51%。结论:采用高效液相色谱法测定结核性胸膜炎患者胸水中的莫西沙星浓度简便、准确、重现性好。Objective: To establish a method for the determination of moxifloxacin concentration in pleural effusion of tuberculous pleurisy patients. Methods: We used high performance liquid chromatography(HPLC) to determine moxifloxacin concentration in pleural effusion of tuberculous pleurisy patients. The determination was performed on Agilent Eclipse XDB-C18 column with mobile phase consisted of acetonitrile-water(0.05% trifluoroacetic acid, 23︰77) at the flow rate of 1.0 m L·min^(-1). UV detection wavelengths were set at 296 nm(0 – 2.5 min), 256 nm(2.5 – 4.5 min) and 296 nm(4.5 – 8.0 min), respectively. The column temperature was 25 ℃ and injection volume was 30 μL. Results: In the range of 0.1 – 10.0 μg·m L^(-1)(r = 0.999 2), moxifloxacin showed a good linear relation. The lower limit of quantitation was 0.1 μg·m L^(-1). The inter-day precision RSD of low, medium and high concentration were 2.76%, 2.23% and 2.00%, and intra-day precision RSD were 5.88%, 3.49% and 3.60%, respectively. The extraction recovery of them were 53.00%, 63.26% and 65.42%, and internal standard was 91.51%, respectively. Conclusion: The measuring method adopted in the experiment can accurately determine the concentration of moxifloxacin in pleural effusion of tuberculous pleurisy patients with convenience, high sensitivity and exceptional reproducibility.

关 键 词：HPLC 莫西沙星 结核性胸膜炎 胸水 药动学 

分 类 号：R969[医药卫生—药理学]