半夏生物碱诱导人肾细胞癌786-0细胞凋亡及其对葡萄糖转运蛋白78表达的调控  

Pinellia Alkaloid Induces Renal Cell Carcinoma 786-0 Cells Apoptosis and Regulaties the Expression of Glucose Transporter Protein 78

作　　者：杨钧显 林黎[2] 高强利 朱保平[3] 

机构地区：[1]武汉大学医学院,湖北武汉430072 [2]武汉市普仁医院泌尿外科 [3]武汉大学中南医院泌尿外科

出　　处：《华南国防医学杂志》2016年第4期231-234,共4页Military Medical Journal of South China

摘　　要：目的观察半夏生物碱对786-0细胞株的凋亡诱导作用,并探讨其可能作用机制。方法将786-0细胞株与半夏生物碱(400、200、100μg/ml)共同培养48 h;细胞计数试剂盒(cell counting kit-8,CCK-8)法测定细胞活性;流式细胞法检测细胞的凋亡率;蛋白免疫印迹法及实时定量聚合酶联反应(polymerase chain reaction,PCR)法检测细胞内葡萄糖转运蛋白78的表达;免疫组织化学法检测细胞中Bad的蛋白表达。结果与阴性对照组比较,干预组细胞活性下降(P<0.01)并发生明显的凋亡现象,差异具有统计学意义(P<0.05);免疫蛋白印迹法及实时定量PCR法检测发现,半夏生物碱干预各组中葡萄糖转运蛋白78表达明显下降(P<0.05);免疫组织化学结果提示,半夏生物碱亦能上调Bad蛋白在细胞内的表达。结论半夏生物碱能通过抑制葡萄糖转运蛋白78的表达,并能上调Bad蛋白的表达,从而促进人肾细胞癌的凋亡。Objective To observe the effect of pinellia alkaloid on inducing 786-0 cell lines apoptosis and to reveal the possible mechanism. Methods 786-0 cell lines were co-cultured with pinellia alkaloid （400,200,100μg/ml） 48 h; the cell viability was determined by cell counting kit-8（CCK-8） assay; the cell apoptosis rate were detected by flow cytometry ;the expression of glucose transporter 78 were detected by western blot and real-time polymerase chain reaction（PCR） ; the expression of Bad protein was detected by immunohistochemstry. Results Compared with the control group, cell activ- ity and promote apoptosis were decreased obviously, and it had a statistic significance（P〈0. 01）; the expression of glu- cose transporter protein 78 were decreased significantly（P〈0. 05）; the expression of Bad protein were Upregulated. Con- clusion Pinellia alkaloids can promote the apoptosis of renal cell carcinoma by inhibiting glucose transporters 78 and up- regulating Bad experssion.

关 键 词：半夏生物碱 人肾细胞癌 细胞凋亡 葡萄糖转运蛋白78 

分 类 号：R285[医药卫生—中药学]