参附药对改善大鼠阳气虚衰型心衰的最佳配比研究  

The Optimal Ratio of Ginseng Compatibility Aconiti to Improve the Yang Qi Deficiency Heart Failure in Rats

作　　者：沈晨薇[1] 张春椿[1] 李效贤[1] 李石清[1] 徐磊[1] 熊耀康[1] 

机构地区：[1]浙江中医药大学,杭州310053

出　　处：《浙江中医药大学学报》2016年第5期389-393,共5页Journal of Zhejiang Chinese Medical University

基　　金：国家自然科学基金项目(81373991)~~

摘　　要：[目的]建立阳气虚衰型心力衰竭(heart failure,HF)大鼠模型,筛选参附药对改善HF的最佳配比,阐明参附药对的配伍与疗效关系。[方法]取术后存活的阳气虚衰型HF模型大鼠,以不同配比参附药对(红参:附子=1:0.1、1:0.5、1:1、1:2、1:10)灌服,同时设置红参组、附子组、手术组和假手术组为对照,采用酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA)测定各组大鼠血清中三碘甲状腺原氨酸(triiodothyronine,T3)、甲状腺素(thyroxin,T4)、促甲状腺素(thyrotropin,TSH)、醛固酮(aldosterone,ALD)、脑钠素(brain natriuretic peptide,BNP)含量。[结果]1:10配比参附药对组比手术组能有效提高大鼠血清T4含量;1:0.5、1:10配比参附药对组均能有效提高大鼠血清TSH含量,同时降低大鼠血清ALD、BNP含量,从而起到治疗阳气虚衰型HF大鼠的作用。[结论]不同配比参附药对对阳气虚衰型HF大鼠具有治疗效果,其中以参附药对1:10组的疗效最佳。[Objective] Establishing heart failure model of Yang Qi deficiency rat, to screen the optimal ratio of Shenfu medicine against heart failure, and to elucidate compatibility and curative effect relation. [Methods] The postoperative survival of Yang Qi deficiency HF rats by gavage with different ratios of Shenfu drugs, was used ELISA method to determine the serum T3, T4, TSH, ALD, BNP levels and operation group, sham operation group as a control group. [Results] 1 ： 10 different proportions of Shenfu medicine compared with operation group could effectively improve the rats serum T4 levels,1 ： 0. 5, 1 ： 10 different proportions of Shenfu medicine compared with operation group could effectively improve the rats serum TSH levels and inhibit the rat serum ALD, BNP levels, which play a role in the treatment of Yang Qi deficiency HF. [Conclusion]Different proportions of Shenfu drug have therapeutic effects on Yang Qi deficiency HF rats, in which the efficacy of Shenfu 1 ： 10 group is best.

关 键 词：阳气虚衰 HF 参附药对 ELISA 

分 类 号：R331[医药卫生—人体生理学]