Genomic insights into the ESBL and MCR-l-producing ST648 Escherichia coli with multi-drug resistance  

作　　者：Huimin Zhang Christopher H. Seward Zuowei Wu Huiyan Ye Youjun Feng 

机构地区：[1]Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou 310058, China [2]Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA [3]Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50010, USA

出　　处：《Science Bulletin》2016年第11期875-878,共4页科学通报（英文版）

基　　金：This work was supported by Zhejiang Provincial Natural Science Foundation for Distinguished Young Scholars （LR15H190001）, the National Natural Science Foundation of China （31570027）, and a start-up package from Zhejiang University （Y.F.）. Dr. Feng is a recipient of the ＂Young 1000 Talents＂ Award.

摘　　要：Polymyxin acts as an ultimate line of refuge against the severe infections by multidrug-resistant Gram- negative pathogens. This conventional idea is challenged dramatically by the recent discovery of mobile colistin resistance gene （mcr-1） is prevalent in food animals and human beings worldwide. More importantly, the mcr-1 gene was found to be co-localized with other antibiotic resistance genes, raising the possibility that super-bugs with pan-drug resistance are emerging. However, little is reported on the genomes of the mcr-l-positive bacterial host reservoirs. Here we report genome sequencing of three human isolates of the mcr-l-positive Escherichia coli （E15004, E15015 and E15017） and define general features through analyses of bacterial comparative genomics. Fur- ther genomic mining together with sequence typing allowed us to elucidate that the MCR-l-carrying E. coli E15017 belongs to the sequence type ST648 and copro- duces extended-spectrum β-1actamase （ESBL）. Given the fact that ST648 has been known to associate New Delhi metallo-β-1actamase 1 or ESBL, with either our results highlighted the possibility of ST648 as an epidemic clone with multidrug resistances.多粘菌素由 multidrug 抵抗的克否定的病原体对严重感染充当避难处的一根最终的线。这个常规想法被活动 colistin 抵抗基因(mcr-1 ) 的最近的发现戏剧性地质问在食物动物和世界范围的人是流行的。更重要地， mcr-1 基因被发现与另外的抗菌素抵抗基因 co 局部性，提起可能性与平底锅药抵抗超级臭虫正在出现。然而，很少在 mcr-1-positive 的染色体上被报导细菌的主人水库。这里，我们报导三个人定序的染色体 mcr-1-positive Escherichia coli (E15004， E15015 和 E15017 ) 孤立并且通过细菌的比较 genomics 的分析定义一般特征。和顺序打字的进一步的 genomic 采矿允许我们阐明 MCR-1-carrying E。coli E15017 属于顺序类型 ST648 和 coproduces 扩大光谱的 -lactamase (ESBL ) 。给 ST648 与任何一个新德里 metallo 为伙伴所知的事实 -- lactamase 1 或 ESBL，我们的结果与 multidrug 电阻作为流行克隆加亮 ST648 的可能性。

关 键 词：MCR-1 Extended-spectrum beta-lactam（ESBL） Colistin resistance ST648 

分 类 号：Q987[生物学—遗传学] S852.612[生物学—人类学]