人原代卵巢肿瘤细胞体外培养的药敏研究及其在临床的应用  

In vitro Chemotherapeutics Susceptibility Studies on Primary Human Ovarian Cancer Cells and Its Clinical Application

作　　者：李樱媚 陈历排[2] 陈孝[1] 

机构地区：[1]中山大学附属第一医院药学部,广东广州510080 [2]广州医科大学附属肿瘤医院妇科肿瘤科,广东广州510095

出　　处：《今日药学》2016年第5期291-294,307,共5页Pharmacy Today

基　　金：广东省科技计划项目(2013A022100024)

摘　　要：目的从卵巢癌患者的肿瘤组织中提取原代肿瘤细胞,建立条件重编程细胞（conditionally reprogrammed cells,CRCs）长期培养体系,并进行相关药敏试验。方法采用胰酶消化法将卵巢肿瘤组织分散成单个细胞后,用普通完全培养基、条件性培养基、条件性培养基及3T3饲养细胞分别培养,观察其生长情况;并对低代数（P2-P3）的肿瘤细胞进行常用化疗药物的药敏试验。结果在条件性培养基及3T3饲养细胞的作用下,原代卵巢肿瘤细胞可以持续增殖,并保持较快增殖速度。药敏试验结果显示,所培养的原代卵巢肿瘤细胞均对顺铂、紫杉醇、多西他赛敏感,与临床结果一致;雷公藤甲素对卵巢癌细胞有明显抑制作用。结论条件重编程（conditionally reprogramming,CR）技术可以作为一种体外快速有效培养原代卵巢肿瘤细胞的方法,将其作为药敏试验模型在肿瘤化疗及耐药机制的研究中具有重要意义。OBJECTIVE To separate primary human ovarian cancer cells from patients with ovarian cancer,establish patientderived conditionally reprogrammed ovarian cancer cells and to do chemotherapeutic drug sensitivity tests. METHODS Trypsin was used to digest the ovarian cancer tissues into single cells. Then the separated cells were cultivated in complete medium,conditional medium with or without 3T3 feeder cell to screen the best medium. The anti-cancer drug sensitivity tests were performed on the low passage（ P2-P3） cancer cells. RESULTS The primary ovarian cancer cells could continue to proliferate in a rapid growth rate in conditional medium with 3T3 feeder cell. And the drug sensitivity tests indicated that the ovarian cancer CRCs were sensitive to cisplatin,paclitaxel and docetaxel,which was consistent with the clinical outcomes. And triptolide can significantly inhibit proliferation of ovarian cancer cells in vitro. CONCLUSION Conditionally reprogramming（ CR） can be used as an efficient method for in vitro culture of primary human ovarian cells and for chemotherapeutic drug sensitivity and resistance investigations.

关 键 词：卵巢癌 原代细胞 条件重编程细胞 药敏试验 

分 类 号：R965[医药卫生—药理学]