取向TSF/PLLA静电纺纳米纤维毡的制备及其生物相容性  

Preparation and Biological Properties of Aligned TSF/PLLA Electrospun Nanofibers

作　　者：邵伟力[1,2,3] 高艳菲[1,2,3] 何建新[2,3] 王倩[2,3] 陈利[1] 崔世忠[1,2,3] 

机构地区：[1]天津工业大学纺织学院,天津300387 [2]中原工学院纺织学院,河南郑州450007 [3]纺织服装产业河南省协同创新中心,河南郑州450000

出　　处：《材料科学与工程学报》2016年第3期384-388,431,共6页Journal of Materials Science and Engineering

基　　金：国家自然科学基金资助项目(51203196;U1204510);NSFC-河南人才培养联合基金资助项目(U1204510);河南省教育厅科学技术研究重点资助项目(14A540003;14A540006);郑州市普通科技攻关资助项目(141PPTGG400)

摘　　要：利用静电纺丝技术制备了取向的柞蚕丝素/左旋聚乳酸(TSF/PLLA)纳米纤维毡。通过扫描电子显微镜(SEM)、X射线衍射分析(XRD)和拉伸测试分别对TSF/PLLA纳米纤维的形貌、结晶结构及力学性能进行了研究。将人成骨肉瘤细胞(MG-63)种植在TSF/PLLA纳米纤维上,通过荧光显微镜分析和MTT(四甲基偶氮噻唑蓝比色法),观察细胞在材料表面的生长情况,评价纳米纤维的生物学性能。结果表明,TSF含量为10%时,纤维直径分布均匀,结晶度高。但是,TSF含量超过10%后,纤维直径粗细不匀明显,纤维的力学性能下降。与无规纤维毡相比,取向的纳米纤维毡力学性能优异,初始模量高,更能够促进细胞增殖,对细胞的生长行为影响大。Aligned TSF（tussah silk fibroin）/Poly l-lactic（PLLA）electrospun nanofibers were successfully fabricated by electrospinning technique.The morphology,secondary structure and mechanical property of the TSF/PLLA nanofibers were characterized by scanning electronic microscope,X-ray diffraction（XRD）and tensile test.Human osteosarcoma cells（MG-63）was cultured in vitro to study the biological properties of TSF/PLLA nanofibers,which was characterized by fluorescence microscope test and four methyl azo thiazole blue colorimetric（MTT）.The results indicated that TSF/PLLA nanofibers had the uniform diameter distribution and high crystallinity when TSF content was 10%.However,the diameters of the fibers were less even and the mechanical properties decreased when the content of TSF was over 10%.Compared with random fiber mat,aligned TSF/PLLA nanofibers showed high mechanical performance and high initial modulus,and we found that aligned TSF/PLLA nanofibers could enhance cell proliferation and had a great effect on cell growth behavior.

关 键 词：柞蚕丝素 聚左旋乳酸 取向纳米纤维 生物学性能 

分 类 号：TS102.1[轻工技术与工程—纺织工程]