慢性心力衰竭大鼠胰岛素通路成分mRNA表达的变化  

mRNA expression of insulin pathway components in CHF rats

作　　者：巫菲 米睿[1] 杨玲[2] 杨帆[1] 李彤[3] 

机构地区：[1]天津医科大学三中心临床学院心脏中心,300170 [2]天津医科大学三中心临床学院内分泌科,300170 [3]天津市第三中心医院心脏中心

出　　处：《中国糖尿病杂志》2016年第6期552-555,共4页Chinese Journal of Diabetes

基　　金：首都医科大学糖尿病防治研究重点实验室项目(2015ZYLB02);天津市卫生局攻关项目(12KG106)

摘　　要：目的观察慢性心力衰竭大鼠肝脏、脂肪及骨骼肌的胰岛素受体(InsR)及其亚型,IRS-1、IRS-2,葡萄糖转运蛋白-1(GLUT-1)和GLUT-4 mRNA的表达及其变化趋势。方法建立腹主动脉缩窄诱导大鼠心力衰竭模型,行腹腔注射胰岛素耐量实验(IPITT),静脉葡萄糖耐量实验(WGTT)和静脉胰岛素释放实验(IRT)。RT-PCR检测各组组织InsR、IRS,GLUT mRNA的表达。结果与假手术组(SHAM)比较,心力衰竭组(CHF)IVGTT、IPITT、IRT结果异常。肝脏和骨骼肌中InsR[(1.02±0.09)vs(2.23±0.33);(0.003±0.000)vs(0.01±0.01),P<0.05]及脂肪中各基因mRNA含量均降低(P<0.05)。结论慢性心力衰竭可引起IR,胰岛素通路成分mRNA发生改变。Objective To investigate the effects of chronic heart failure（CHF） on mRNA expressions of insulin receptor（InsR）and its subtypes,insulin receptor substrate（IRS）-1 and 2,and glucose transporter（GLUT）-1 and 4 in rat livers,fats and skeletal muscles.Methods CHF model was established by inducing coarctation of abdominal aorta in rats.Tests of intraperitoneal insulin tolerance（IPITT）,intravenous glucose tolerance（IVGTT） and intravenous insulin release（IRT）were performed.＇RT-PCR and real-time PCR were used to assess the mRNA expressions of InsR,IRS and GLUT in various tissues.Results Compared with the control group,the values of IVGTT,IPITT,and IRT were obviously abnormal in the CHF group.The mRNA expression levels of InsR gene in liver and skeletal muscles[（1.02±0.09）w（2.23±0.33）;（0.003±0.000） vs（0.01±0.01）,P〈0.05]and the expressions of all genes studied in fats in the CHF group were decreased significantly（P〈0.05）.Conclusion CHF may result in insulin resistance and abnormal mRNA expressions in various tissues.

关 键 词：慢性心力衰竭 胰岛素抵抗 大鼠 

分 类 号：R541.6[医药卫生—心血管疾病]