手机频率电磁辐射对大鼠睾丸超微结构损伤的研究  

Cellphone electromagnetic radiation damages the testicular ultrastructure of male rats

作　　者：曹晓慧[1] 马惠荣[1,2,3] 马雪莲[2] 陈杰[1] 张国红[4] 

机构地区：[1]河北医科大学研究生学院,河北石家庄050017 [2]河北中医学院中西医结合生殖疾病协同创新中心,河北石家庄050200 [3]河北省中西医结合肝肾病证重点实验室,河北石家庄050091 [4]河北中医学院基础医学院,河北石家庄050200

出　　处：《中华男科学杂志》2016年第6期491-495,共5页National Journal of Andrology

基　　金：河北省人口和计划生育委员会计划生育科研项目(2013-A15)~~

摘　　要：目的:观察手机频率(900 MHz)电磁辐射对雄性大鼠睾丸组织超微结构和生精细胞凋亡的影响。方法:将30只SPF级健康雄性SD大鼠随机分为3组:正常组、辐射2 h组和辐射4 h组,每组10只。正常组不辐射,辐射2 h组和辐射4 h组每天暴露于900 MHz手机频率分别辐射2、4 h,连续辐射30 d。透射电镜观察睾丸组织超微结构的变化,原位末端标记(TUNEL法)检测生精细胞凋亡。结果:与正常组比较,辐射2 h组大鼠生精小管基膜肿胀,支持细胞紧密连接分开,细胞间隙明显增大,部分线粒体内出现空泡,见线粒体髓样化,生精细胞凋亡增多[(25.62±1.33)%vs(18.71±1.76)%,P<0.05],以初级精母细胞凋亡为主;与辐射2 h组比较,辐射4 h组生精小管基膜肿胀,支持细胞紧密连接分开,细胞间隙明显增大,精原细胞胞膜不完整,可见胞质碎片、胞核固缩和切迹,核周隙轻度扩张,细胞内线粒体畸形,内有空泡,结构模糊,部分嵴融合或消失,其损伤程度增加,生精细胞凋亡增多[(29.93±1.46)%vs(25.62±1.33)%,P<0.05],精原细胞、初级精母细胞和次级精母细胞均有凋亡。结论:手机频率电磁辐射可造成大鼠睾丸组织超微结构损伤,生精细胞凋亡增加,有时效关系;生精细胞凋亡可能与线粒体损伤有关。Objective： To investigate the influence of cellphone electromagnetic radiation（CER） on the testicular ultrastructure and the apoptosis of spermatogenic cells in male rats. Methods： Thirty adult male SD rats were equally randomized into a 2 h CER,a 4 h CER,and a normal control group,the former two groups exposed to 30 days of 900 MHz CER for 2 and 4 hours a day,respectively,while the latter left untreated. Then the changes in the ultrastructure of the testis tissue were observed under the transmission electron microscope and the apoptosis of the spermatogenic cells was determined by TUNEL. Results： Compared with the normal controls,the rats of the 2 h CER group showed swollen basement membrane of seminiferous tubules,separated tight junction of Sertoli cells,increased cell intervals,apparent vacuoles and medullization in some mitochondria,and increased apoptosis of spermatogenic cells,mainly the apoptosis of primary spermatocytes（ P〈0. 05）. In comparison with the 2 h CER group,the animals of the 4 h CER group exhibited swollen basement membrane of seminiferous tubules,more separated tight junction of Sertoli cells,wider cell intervals,incomplete membrane of spermatogonial cells,fragments of cytoplasm,nuclear pyknosis and notch,slight dilation of perinuclear space,abnormalities of intracellular mitochondria with vacuoles,fuzzy structure,and fusion or disappearance of some cristae,and increased damage of mitochondria and apoptosis of spermatogenic cells,including the apoptosis of spermatogonial cells,primary spermatocytes,and secondary spermatocytes（ P〈0. 05）. Conclusion： CER can damage the testicular ultrastructure and increase the apoptosis of spermatogenic cells of the male rat in a time-dependent manner,and the apoptosis of spermatogenic cells may be associated with the damage to mitochondria.

关 键 词：手机 电磁辐射 雄性大鼠 睾丸超微结构 细胞凋亡 

分 类 号：R594.8[医药卫生—内科学]