杂种蓝莓(野生ב美登’)组培快繁  

Rapid Propagation System of Hybrid Blueberry( wild בBlomidon')

作　　者：甄成[1] 毕显禹[1] 李淑娟[1] 孙小环 许艳玲 石德山 

机构地区：[1]林木遗传育种国家重点实验室(东北林业大学),哈尔滨150040 [2]大兴安岭地区农业林业科学研究院

出　　处：《东北林业大学学报》2016年第5期29-33,共5页Journal of Northeast Forestry University

基　　金：林木遗传育种国家重点实验室(东北林业大学)创新项目(2015B03);大兴安岭行署科技局博士后项目资助(2012A02)

摘　　要：为了建立高效的杂种蓝莓组培快繁技术体系,以大兴安岭地区野生蓝莓和栽培品种‘美登’(‘Blomidon’)杂交获得的杂交种子为试材,筛选出杂交种子最佳无菌处理条件,获得蓝莓杂种无菌苗。再以杂种无菌苗茎段为外植体,探究了不同质量浓度植物生长调节剂(ZT)、蔗糖及继代周期对丛生芽诱导的影响,并探讨了培养基种类及植物生长调节剂IBA质量浓度对生根诱导的影响。结果表明:杂交种子经75%酒精和3%次氯酸钠分别处理20 s和7 min效果最好,污染率为0;添加0.6 mg·L^(-1)ZT和20 g·L^(-1)蔗糖的WPM培养基为丛生芽诱导最佳培养基,诱导率高达100%,且芽丛密集、长势好;芽诱导期间,30 d的继代周期最有利于丛生芽的增殖,增殖倍数高达34.72;最佳生根培养基为添加0.4 mg·L^(-1)IBA的1/2WPM培养基,生根率高达88%,且生根周期仅为40 d,移栽成活率达到85%以上。The objective of the study is to establish a rapid propagation system of hybrid blueberry. The wild blueberry of Daxing＇an Mountains and the cultivar of ‘Blomidon＇were selected as parents to take intervarietal crosses and the hybrid seeds. The best sterile treatment conditions for hybrid seeds were selected and the sterile seedlings were got. The effects of the concentration of ZT,the contents of source and subculture cycle on frequency of cluster buds induction were conducted by using stem segments of seedlings as explants. The influence of different types of medium and concentration of IBA on the induction of adventitious root was analyzed. The contamination rate of hybrid seeds was 0 after sterilized with 75% ethanol and 3% sodium hypochlorite for 20 s and 7 min,respectively. The best medium for cluster buds induction was WPM containing 0.6 mg·L（-1）ZT and 20 g·L（-1）source. The induction rate was up to 100% and the cluster buds grew well. During the bud induction,the optimal subculture cycle for buds proliferation was 30 d with the proliferation coefficient of 34.72. The best rooting medium was 1 / 2WPM containing 0.4 mg·L（-1）IBA,the rooting rate was 88%,and the rooting period was only40 d. The survival rate of transplanting was more than 85%.

关 键 词：蓝莓 野生×'美登’ 杂交种子 组织培养 快速繁殖 

分 类 号：Q943.1[生物学—植物学]