Fe3O4纳米粒子与慢病毒载体共同感染内皮祖细胞的促血管新生和MRI示踪的应用  

Preparation of Fe_3O_4 Nanoparticles and Lentiviral Vector Infected EPCs for the Improvement of Angiogenesis and MRI Tracing

作　　者：王娟[1] 周兵[2] 王欣欣[3] 吴秀娟[1] 厉婷[1] 张文科[3] 刘亢丁[1] 

机构地区：[1]吉林大学第一医院,长春130021 [2]吉林大学化学学院物理化学系,长春130012 [3]超分子结构与化学国家重点实验室,吉林大学化学学院,长春130012

出　　处：《高等学校化学学报》2016年第6期1148-1153,共6页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:81271294)资助~~

摘　　要：采用化学共沉淀法制备了柠檬酸钠修饰Fe_3O_4纳米粒子(NPs),使用胎牛血清(FBS)改善Fe_3O_4NPs的分散性.实验表明Fe_3O_4NPs尺寸均匀,且具有良好的稳定性,FBS浓度小于5%(体积分数)时,Fe_3O_4NPs无聚集沉淀;在300 K下,饱和磁化强度达到74.86×10^(-3)A·m^2/g(74.86 emu/g);核磁共振T2序列成像时,75μg/m L Fe_3O_4NPs与慢病毒载体(LV)共同标记内皮祖细胞(EPCs)成像效果良好;而且EPCs具有稳定过表达目的基因血管内皮生长因子(VEGF)的能力.利用Fe_3O_4NPs与LV共同感染EPCs,可有效促进大鼠血管生成.说明修饰后的EPCs兼具核磁共振成像(MRI)示踪和促血管生成双重功能.Fe3O4nanoparticles（ NPs） modified with sodium citrate were prepared via chemical coprecipitation method and fetal bovine serum（ FBS） could improve the dispersibility of Fe3O4 NPs in FBS solution（ volume fraction≤5%）. At the temperature of 300 K,the saturation magnetization intensity of NPs reached 74. 86 ×10^-3A·m^2/ g（ 74. 86 emu / g）; MRI T2 imaging of endothelial progenitor cells（ EPCs） became better after being infected by 75 μg / m L of Fe3O4 NPs and lentiviral vectors（ LV）; furthermore,EPCs could stably over express VEGF target gene. EPCs co-infected with Fe3O4 NPs and LV,after being injected into caudal vein of focal cerebral ischemia rats could effectively promote angiogenesis. These results indicated the modified EPCs possessed both MRI tracing and angiogenesis,and therefore had potential application value in clinical therapy of cerebral ischemia.

关 键 词：FE3O4纳米粒子 内皮祖细胞 慢病毒载体 血管内皮生长因子 磁共振成像 

分 类 号：O631[理学—高分子化学] O647[理学—化学]