不同供体对玉米自交系QR273的改良效应  

Improvement Effects of Donors on Inbred Line QR273 in Maize

作　　者：郭向阳 陈泽辉 胡兴 兰琴英[1,3] 祝云芳 王安贵 陈建军[1,3] 任洪 

机构地区：[1]贵州省旱粮研究所,贵阳550006 [2]四川华龙种业有限责任公司,成都610400 [3]贵州大学农学院,贵阳550025

出　　处：《玉米科学》2016年第3期15-19,共5页Journal of Maize Sciences

基　　金：优良热带玉米Suwan群体遗传潜势及种质改良创新研究[黔科合NZ(2013)3004号];贵州玉米育种核心种质的改良与创制[黔农科院自主创新科研专项(2014)006号]

摘　　要：基于田间试验统计和SSR标记的遗传多样性分析,分析4个供体对优良热带玉米自交系QR273改良效应。结果表明,不同供体间的6个性状在P=0.05水平上存在显著差异,以自交系QB572、QB576和T32为供体,改良系与QR273在穗长性状上有不同程度的减少;在穗粗和穗行数上多数改良系得以提高。不同供体大多数性状的表型变异系数变化幅度小,比对照增产10%以上的组合均由供体QB576和T32所提供。基于SSR分子标记分析结果,从146对引物中用筛选出的清晰稳定的引物42对,对33份供试材料进行同源性位点扩增,共检测到162个等位基因变异,平均每个点检测到等位基因数位3.9个,变化范围1-6个。每个位点的PIC值变幅为0.079 5-0.515 9,平均为0.283 8。通过UPGMA和主坐标分析,将供试材料被划为4个类群,第Ⅰ类群包括除QB2147、QB2188、QB1955、QB2163和QB2164外其他QR273改良系;第Ⅱ类群为QB2147;第Ⅲ类群为QB2188、T32、QB572和QB576;第Ⅳ类群为QB1955、QB2163、QB2164和QB48。Based on the field test statistics and genetic diversity with SSR markers, the improvement effects offour donors played on inbred line QR273 were analyzed. The results showed that significant difference（P=0.05） ex-isted between different donors in 6 traits. In inbred lines QB572, QB576 and T32 for the donor, improved lines andQR273 showed different degrees of reduction on ear length, ear diameter and ear rows had improved in most im-proved lines. Phenotypic trait variation coefficients in the most donors change little in general. Control more than10% of the measured cross combinations thereof, are donor QB576 and T32 the group with a combination of im-proved lines, improved significantly. SSR marker analysis based on the results from 146 pairs of primers werescreened using primers clear and stable member 42 right, right for 33 inbred lines were homologous loci amplified162 alleles were detected variation, the average number of alleles detected at each point position 3.9, range 1-6.PIC value of the amplitude of each site is 0.079 5-0.515 9, with an average of 0.283 8. By UPGMA and principal co-ordinates analysis, test inbred classified into four groups： the first group included Ⅰ except QB2147, QB2188,QB1955, QB2163 and QB2164 alia QR273 improved lines; the first Ⅱ groups were QB2147; the first groups of ⅢQB2188, T32, QB572 and QB576; section Ⅳ groups were QB1955, QB2163, QB2164 and QB48.

关 键 词：玉米 供体 自交系 SSR标记 改良效应 

分 类 号：S513.035.1[农业科学—作物学]