响应面法优化大豆根瘤菌HW-05保护剂配方  

Optimization of Protective Agent Formulation of Rhizobium HW-05 by Response Surface Methodology

作　　者：甄涛[1] 陈薇[2] 沙长青[2] 张先成[1] 刘新杰[3] 于徳水 

机构地区：[1]黑龙江省科学院微生物研究所,黑龙江哈尔滨150010 [2]黑龙江省科学院,黑龙江哈尔滨150000 [3]黑龙江省科学院高技术研究院,黑龙江哈尔滨150020

出　　处：《安徽农业科学》2016年第10期3-5,8,共4页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省科学院科学研究基金项目

摘　　要：[目的]采用响应面法研究具有根瘤菌保护作用的蛋白类、盐类、糖类和微量元素类物质。[方法]通过设置不同种类、组合、配比、浓度的大豆根瘤菌保护剂配方,在实验室中模拟实际应用条件,研究具有提高根瘤菌存活率和存活时间作用的液体保护剂配方。采用响应面法的中心组合试验确定各显著因子的最佳水平。[结果]筛选出各类化合物中影响有效活菌数的显著因子,即蛋白胨、黄原胶、Na Cl优化后的各类化合物的终浓度如下:蛋白胨0.13%,黄原胶0.011%,氯化钠0.30%。[结论]菌株HW-05在加入保护剂室温放置180 d后的有效活菌数为3.185×108CFU/m L,与未加入保护剂优化前(2.458×108CFU/m L)相比存活率提高了25%以上。[Objective] The response surface methodology was used to study the protective effect of proteins,salts,sugars and trace elements on Rhizobium. [Method] Through setting up different types,combinations,ratios and concentrations of Rhizobium protective agent formulation,simulating actual conditions in laboratory,liquid protective agent formulation with effect of improving survival rate and time of Rhizobium was studied. The optimal level of each significant factor was determined by using central composite experiment of response surface methodology. [Result] The significant factors influencing the number of effective viable bacteria in compounds were obtained,namely peptone,xanthan gum and Na Cl; The optimized final concentrations of compounds were： peptone 0. 13%,xanthan gum 0. 011% and Na Cl 0. 30%.[Conclusion]The effective viable count of HW- 05 strain is 3. 185 × 108 CFU / m L after addition of protective agent at room temperature for180 d,compared with the previous（ 2. 458 × 108 CFU / m L） protective agent is not added,the survival rate is increased by more than 25%.

关 键 词：大豆根瘤菌 保护剂配方 优化 响应面法 

分 类 号：S501[农业科学—作物学] Q781[生物学—分子生物学]