小窝蛋白-1对MRL／lpr狼疮鼠肾损伤及肾小球紧密连接蛋白的影响  

Effect of caveolin-1 on renal injury and the expression of tight junction protein in MRL/Ipr mice kidney

作　　者：冯欣[1] 高薇[1] 

机构地区：[1]辽宁医学院附属第一医院风湿免疫科,锦州121000

出　　处：《中华风湿病学杂志》2016年第6期364-367,共4页Chinese Journal of Rheumatology

基　　金：辽宁医学院校长基金（XJZZ20130255）

摘　　要：目的探讨小窝蛋白（caveolin）-1对MRL/lpr狼疮小鼠肾损伤及肾小球紧密连接蛋白的作用。方法实验分为4组：正常对照组（BALB/c小鼠）：5只；MRL/lpr自发性狼疮小鼠（18只）采用随机数字表法分为：模型组，阴性对照组即空白载体组，caveolin-1转染组，每组6只。检测小鼠尿蛋白定量（24 h）、血尿素氮、肌酐水平的变化。蛋白印迹法检测各组小鼠肾组织中caveolin-1蛋白和紧密连接蛋白occludin，claudin-5及ZO-1的表达水平。采用单因素方差分析和LSD-t检验进行统计学分析。 结果与对照组相比，模型组小鼠尿蛋白定量（24 h）[（2 894±437）mg，（412±72）mg]、尿素氮[（8.7±1.5）mmol/L，（6.9±0.4）mmol/L]、肌酐[（106±22）μmol/L，（85±4）μmol/L]均明显上升，caveolin-1蛋白表达水平升高（265±17，61±6），occludin（114±12，190±12），claudin-5（60±5，80±6）及ZO-1（98±11，206±15）蛋白的表达水平降低（P〈0.05）。caveolin-1转染后，小鼠的尿蛋白定量（24 h）[（1 253±249）mg，（2 894±437）mg]、尿素氮[（6.5±1.3）mmol/L，（8.7±1.5）mmol/L]、肌酐[（78±17）μmol/L，（106±22）μmol/L]水平均明显下降，occludin（218±16，114±12），claudin-5（87±6，60±5）及ZO-1（313±17，98±11）蛋白的表达水平升高（P〈0.05）。结论Caveolin-1蛋白在LN肾组织的表达升高，通过降低紧密连接蛋白的表达，参与LN的发病。Objective To study the effect of caveolin-1 on renal injury and the expression of tight junction proteins in MRL/lpr mice kidney. Methods The mice were divided into 4 groups： 5 mice in the normal control group （BALB/c mice）; the MRL/lpr lupus mice （n=18） were randomly divided into the MRL/lpr group in which 6 mice were included; the negative control group in which 6 mice were included; the caveolin-1 transfection group in which 6 mice were included. The changes of urine protein, the levels of urea （BUN） and creatinine （Cr） were detected. The expressions of claudin-5, occludin, ZO-1 and caveolin-1 protein were determined by western bloting. Analysis of variance was used to determine statistical significant differences between the two groups. A significance level of 0.05 was considered as signigicant.Results Compared with the control group, 24 h urine protein [（2 894±437） mg, （412±72） mg], BUN [（8.7±1.5） mmol/L, （6.9±0.4） mmol/L], Cr [（106±22） μmol/L, （85±4） μmol/L] were significantly increased, level of caveolin-1 protein increased （265±17, 61±6）, the level of occludin （114±12, 190±12）, claudin-5 （60±5, 80±6） and ZO-1 （98±11, 206±15） protein decreased in the MRL/lpr group （P〈0.05）. After caveolin-1 transfection, the levels of urinary protein [（1 253±249） mg, （2 894±437） mg], BUN [（6.5±1.3） mmol/L, （8.7±1.5） mmol/L], Cr [（78±17） μmol/L, （106±22） μmol/L] were significantly decreased, and the levels of occludin （218±16, 114±12）, claudin-5 （87±6, 60±5） ZO-1 （313±17, 98±11） were increased （P〈0.05）. Conclusion The expression of caveolin-1 protein in the renal tissues of lupus nephritis increases. Caveolin-1 can reduce the expression of tight junction proteins and contribute to progres-sion of lupus nephritis.

关 键 词：狼疮肾炎 CAVEOLIN-1 紧密连接蛋白 

分 类 号：R593.242[医药卫生—内科学]