白术对脑老化小鼠记忆能力和突触结构的影响  

Effect of Rhizoma Atractylodis Macrocephalae on Learning-memory and Synaptic Structure in Aging Mice

作　　者：高群[1] 于新宇[2] 

机构地区：[1]菏泽医学专科学校,山东菏泽274000 [2]大连大学医学院,辽宁大连116000

出　　处：《菏泽医学专科学校学报》2016年第2期1-3,14,共4页Journal of Heze Medical College

摘　　要：目的探讨白术对D-半乳糖致脑老化小鼠学习记忆及海马突触结构的影响。方法采用连续皮下注射D-半乳糖的方法,建立脑老化小鼠模型,通过Morris水迷宫检测小鼠的记忆能力,用透射电镜和形态计量法分析白术对脑老化小鼠CA3区Gray I型突触结构参数的影响。结果水迷宫实验:脑老化组的逃避潜伏期显著长于对照组P<0.05,而RAM低、中、高组的逃避潜伏期显著短于脑老化组(P<0.05,P<0.01。海马CA3区Gray I型突触结构观察:与对照组相比,脑老化组小鼠的突触界面曲率、突触后致密物厚度均显著减小(P<0.05;P<0.01),突触间隙宽度显著增大(P<0.05);白术组小鼠的突触界面曲率、突触间隙宽度、突触后致密物厚度与对照组基本相近似(P>0.05)。结论白术可以改善脑老化所致小鼠海马CA3区Gray I型突触结构的改变,改善学习记忆能力。Objective To observe the effects of rhizoma atractylodis macrocephalae（RAM） on learning-memory and synaptic structure in D-galactose-induced brain aging mice. Methods D-galactose was injected subcutaneously for the brain aging model. Learning-memory ability was tested by Morris water maze test and The structure of Grey I type synapses in hippocampal CA3 area were observed under transmitted electron microscope. Results Morris water maze test： In brain aging group, the escape latency was significantly prolonged,（P〈0.05）. The escape latency in 3 RAM group was significantly shorter（P〈0.05,P〈0.01）. The structure of Grey I type synapses in hippocampal CA3 area. In brain aging group, the curvature of synapse interface and thickness of postsynaptic desity were significantly decreased（P〈0.05, P〈0.01）, and the width of synaptic cleft was significantly increased（P〈0.05） compared with control group. In RAM-treated groups, the curvature of synapse interface, width of synaptic cleft and thickness of postsynaptic desity were approximately the same as that of control group（P〈0.05）. Conclusion RAM could significantly increase the ability of learning-memory in brain aging mice. the mechanism related to the protective effect on synapese in hippocampal CA3 area.

关 键 词：白术 脑老化 学习记忆能力 突触 超微结构 

分 类 号：R28[医药卫生—中药学]