SELDI-TOF-MS技术预测癌痛发生的研究之一——采用SELDI-TOF-MS技术捕获癌痛的指纹标记  

One of the series of studies on the prediction of cancer pain by SELDI-TOF-MS( Capture the the fingerprint marker of cancer pain by SELDI-TOF-MS)

作　　者：吉利娜 魏亮[2] 裴毅 

机构地区：[1]山西大医院,山西太原030032 [2]山西医科大学第一医院,山西太原030001

出　　处：《临床医药实践》2016年第6期406-409,共4页Proceeding of Clinical Medicine

基　　金：山西省自然基金项目(项目编号:2011011038-3)

摘　　要：目的:借助表面增强激光解吸电离飞行时间质谱(SELDI-TOF-MS)技术捕获晚期恶性肿瘤癌痛发生时的指纹标记。方法:选择24例经病理学或细胞学确诊的晚期恶性肿瘤患者,清晨采集空腹末梢静脉血,采血后观察3个月。将3个月内发生癌痛的患者的血清样本归为癌痛组(9例),3个月内未发生癌痛的血清样本归为无痛组(15例),应用CM10弱阳离子芯片结合SELDI-TOF-MS技术检测两组患者血清样本的蛋白质谱。结果:无痛组与癌痛组相比有6个蛋白质峰的差异有统计学意义(P<0.05),蛋白质质荷比(M/Z)分别为2 755,8 678,8 813,11 361,11 447,11 605。其中与无痛组相比,癌痛组上调的峰M/Z为2 755,11 361,11 447,11 605,下调的峰M/Z为8 678和8 813。进一步对所有患者的蛋白质指纹图谱进行分析,得出:M/Z为2 755,8 678,8 813的蛋白质组在两组之间都存在,癌痛组其丰度中位值分别为6,2,3,无痛组中其丰度中位值分别为2,6,10,而M/Z为11 361,11 447,11 605在癌痛组中呈阳性表达,无痛组则呈阴性表达。结论:蛋白质M/Z 2 755高表达,M/Z 8 678,8 813低表达,M/Z 11 361,11447,11 605呈阳性表达,可以作为癌痛发生的特异指纹标记。Objective： To capture the fingerprint marker of advanced malignant neoplasm when pain occurs. Methods：Chose 24 patients with advanced malignant neoplasm confirmed by pathology or cytology,collected peripheral venous blood on an empty stomach,followed up till three months later,they were divided into 2 groups according to the pain occurrence,pain group（ 9 cases） and painless group（ 15 cases）,by SELDI- TOF- MS（ surface- enhanced laser desorption / ionization time-of- flight mass spectrometry） technique and CM10 proteinchip,these serum proteomic fingerprints were memorized. Results：There were 6 significant different protein fingerprints between painless and pain group,M / Z values were 2 755,8 678,8 813,11 361,11 447,11 605. Compared with painless group,M / Z2755,11 361,11 447,11 605 were up- regulated expressed,and 8678,8 813 down- regulated expressed. We draw the following results from further analysis： M / Z 2 755,8 678,8 813 were present between the two groups,the median value was 6,2,3 in the pain group,and 2,6,10 in the painless group. In addtion,M / Z11 361,11 447,11 605 in positive expression of pain,painless group were negative. Conclusion： The protein fingerprints of M /Z2755 high protein expression,M / Z8 678,8 813 lower expression,M / Z11 361,11 447,11 605 positive expression can exist as the special fingerprint marker of cancer pain.

关 键 词：SELDI-TOF-MS技术 癌痛 晚期恶性肿瘤 

分 类 号：R441.1[医药卫生—诊断学]