接种菌剂对稻秸好氧厌氧两相发酵产气影响  

Effect of microbial inoculants on combined aerobic and anaerobic twophase fermentation of rice straw for biogas production

作　　者：李文哲[1] 丁清华[1] 罗立娜[1] 范金霞[1] 李鹏飞[1] 王金秋[1] 

机构地区：[1]东北农业大学大学工程学院,哈尔滨150030

出　　处：《东北农业大学学报》2016年第5期98-105,共8页Journal of Northeast Agricultural University

基　　金："十二五"农村领域国家科技计划项目(2014BAD02B04)

摘　　要：为提高水稻秸秆在好氧厌氧两相发酵工艺中降解率,采用好氧水解阶段添加不同菌剂方法提高好氧产酸相纤维素降解率。结果表明,各处理组中木霉和黑曲霉混菌组较单一菌种组纤维素和半纤维素降解率更高,水解效果更好。其中木霉黑曲霉混菌曝气组,总酶活最高,纤维素和半纤维素降解效果最好,累积产甲烷量最高,TS产气量最高达到438.57 m L·g-1。由此可见,木霉、黑曲霉混菌并同时曝气供气方式能显著提高水稻秸秆固体有机物降解利用率,促进秸秆生物质转化为沼气。In order to improve the degradation rate of rice straw in the combined aerobic and anaerobic fermentation process, different microbial inoculants were employed to improve the degradation rate of cellulose in the aerobic hydrolysis stage. The results showed that the mixed strains could obtain better cellulosic degradation than the applied with single strain in each treatment. The treatment of Aspergillus mixed with Trichoderma by aeration gas supply obtained the best total cellulase activity, degradation rate of cellulose and cumulative biogas yield of 438.57 m L · g- 1. Therefore, the degradation rate of rice straw could be improved by adding mixed strains of Aspergillus and Trichoderma to the aerobic stage by aeration gas supply.

关 键 词：木霉 黑曲霉 纤维素降解 酶活 产气特性 

分 类 号：S216.4[农业科学—农业机械化工程]