三峡库区消落带两种草本植被土壤细菌群落多样性  

Evaluation of bacterial diversity under different herb vegetation types in the hydro-fluctuation zone of the Three Gorges Reservoir in China

作　　者：任庆水 马朋[1] 李昌晓[1] 秦红[1] 杨予静[1] 

机构地区：[1]西南大学生命科学学院三峡库区生态环境教育部重点实验室,重庆400715

出　　处：《生态学报》2016年第11期3261-3272,共12页Acta Ecologica Sinica

基　　金：重庆市林业重点科技攻关项目(渝林科研2015-6);国家科技部国际合作专项(2015DFA90900);重庆市基础与前沿研究计划重点项目(CSTC2013JJB00004);中央高校基本科研业务费专项资金(XDJK2013A011);重庆市研究生科研创新项目(CYS2015068)

摘　　要：在三峡库区消落带植被重建区域,选取两种人工草本植被—牛鞭草与狗牙根(处理组),并以裸地土壤为对照,研究植被修复与重建对土壤细菌群落多样性的影响。采用分子生物学T-RFLP技术,分别于2012年7月(T1)、2013年7月(T2)及2014年7月(T3)进行原位取样,对消落带人工植被牛鞭草、狗牙根及裸地土壤细菌群落多样性进行了研究。结果表明:(1)除HhaⅠ酶切结果中,物种因素对消落带土壤细菌Shannon-Wiener指数(H)、时间因素对土壤细菌Simpson指数(D)无显著影响外,采用两种酶切之后(HhaⅠ酶、MspⅠ),物种、时间、以及两者的交互作用均对三峡库区消落带土壤细菌群落多样性指数产生显著影响;(2)HhaⅠ酶切后人工植被土壤丰度与裸地相比显著升高,此外,两种酶切结果均表明,人工植被土壤细菌群落多样性指数与裸地相比更加稳定。(3)植被类型、取样时间不同,土壤中的优势片段种类与丰度均不相同;(4)CCA分析结果表明三峡库区消落带土壤细菌群落多样性与化学性质关系密切。研究表明牛鞭草与狗牙根栽植后土壤细菌群落多样性指数的更加稳定,三峡库区进行植被恢复与重建值得肯定。To better understand the growth condition of vegetation,our study was conducted to explore the dynamics of the soil bacterial community in the hydro-fluctuating zone of the Three Gorges Reservoir（TGR） region in China.In July（2012,2013,and 2014）.We collected and compared soil samples from under two plant species（Hemarthria compressa and Cynodon dactylon） and bare soil.We characterized bacterial community diversity using the terminal-restriction fragment length polymorphism（T-RFLP） molecular method,and also investigated soil chemical properties.Our results show that（1） bacterial diversity was significantly influenced by plant species,time,and their interaction,regardless of which enzyme was used for digestion（HhaⅠ or Msp I）;however,species for the Shannon-Wiener index（H） and time for the Simpson index（D） when digested by HhaⅠ did not significantly influence bacterial diversity.（2） The richness index（S） of the planted vegetation was significantly higher than in bare areas.In addition,the S,H,D,and evenness（E）indices were more stable for bacterial communities under planted vegetation than bare areas.（3） Vegetation and sampling times influenced the types and relative abundance of predominant terminal restricted fragments in our study.（4） CCA（canonical correspondence analysis） analysis showed that long fragments were more concentrated,especially around nitrogen and phosphorus,and soil bacterial diversity had a close relationship with chemical properties,such as nitrogen,phosphorus and organic matter.In conclusion,two different enzymes were used to explore the diversity of the soil bacterialcommunity in the TGR region,and their digestion maps,analyzed by synthesis,illustrate that our analysis results are objective.Furthermore,the results indicate that vegetation type and soil chemical properties affect diversity in the soil bacterial community.However,in the fluctuation zone of the TGR,the bacterial diversity indices became more stable after revegetation,which

关 键 词：T-RFLP 三峡库区 消落带 植被修复 细菌群落多样性 

分 类 号：S154.3[农业科学—土壤学]