HPLC法测定炎热清片中黄芩苷、黄芩素和汉黄芩素的含量  

Simultaneous determination of baicalin,baicalein and wogonin in Yan Reqing Tablets by HPLC

作　　者：康珊芬 

机构地区：[1]舟山市市场监督管理局定海分局,浙江定海316000

出　　处：《浙江医学教育》2016年第3期45-47,共3页Zhejiang Medical Education

摘　　要：目的:建立高效液相色谱法测定炎热清片中黄芩苷、黄芩素和汉黄芩素的含量。方法:以WATERS XBridge C_(18)(250mm×4.6mm,5μm)为色谱柱,甲醇-0.1%磷酸溶液为流动相,梯度洗脱,流速1.0ml/min,柱温30℃,检测波长277nm。结果:色谱峰分离度良好,黄芩苷在0.1118~1.118μg、黄芩素在8.225×10^(-3)~8.225×10^(-2)μg、汉黄芩素在3.975×10^(-3)~3.975×10^(-2)μg内呈良好线性关系。平均加样回收率分别为98.8%、97.9%和97.4%。结论:该方法简单、快速、重复性好,可用于炎热清片中黄芩苷、黄芩素和汉黄芩素的质量控制。[Objective]To establish a method for determining the content of baicalin,baicalein and wogonin in Huatan Pingchuan tablets by HPLC. [Method]WATERS X- Bridge C18（ 250 mm × 4. 6mm,5μm） was used. The mobile phase was consisited of methanol- 0. 1% phosphoric acid by gradient elution. The flow rate was 1. 0 ml /min. The column temperature was set at 30℃. The detector wavelength was 277 nm. [Result]Baicalin,baicalein and wogonin showed a good linear relationship with range in 0. 1118 ～ 1. 118μg,8.225×10^-3～8.225×10^-2μg,and 3.975×10^-3～3.975×10^-2μg,respectively. The recovery were 98. 8%,97. 9%,and 97. 4%. [Conclusion]The method is simple,rapid,accurate and reliablewhich can be applied to control the quality of Yan Reqing tablets.

关 键 词：HPLC 炎热清片 黄芩苷 黄芩素 汉黄芩素 

分 类 号：R917[医药卫生—药物分析学]