红外光谱法快速鉴别不同产地中药党参的研究  

Quick Identification of Chinese Traditional Medicine Codonopsis Radixfrom Different Places by Infrared Spectrometry

作　　者：陈前锋[1] 侯鹏[1] 刘巧[1] 马小娅[1] 李攀[1] 祝慧凤[1] 

机构地区：[1]西南大学药学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2016年第6期188-194,共7页Journal of Southwest University(Natural Science Edition)

基　　金：西南大学校地合作项目(104290-41003012);西南大学博士基金项目(SWU111076);西南大学药学实验教学中心资助的创新实验项目;2013年重庆高校创新团队建设计划资助项目;中央高校基本科研业务费创新团队项目(XDJK2013A025);中央高校基本科研业务费项目(XDJK2013C145)

摘　　要：以不同产地中药党参样品的红外指纹图谱为依据,利用共有峰率和变异峰率两个指标,计算并建立所测样品的共有峰率和变异峰率双指标序列,同时结合二阶导数谱进一步放大分析,探讨不同产地和品种党参的相似度并进行快速鉴别.结果表明：4种不同产地的川党参共有峰率均大于71.4%,5种不同产地党参共有峰率最高达到83.3%,最低为55.0%;川党参与党参之间共有峰率最高达到94.4%,最低为59.1%;素花党参与不同产地的川党参与党参的共有峰率均大于65.0%.不同产地和品种的党参随着种质资源的变化,化学成分也会发生相应的变化,在红外光谱指纹图谱中均有差异,在具有高分辨力的二阶导数谱1 800-400cm-1波段区分更加明显.傅里叶变换红外光谱法结合二阶导数能从整体上分析谱图特征峰的变化规律,可以解决中药品质差异和产地归属的问题,为党参药材的质量控制提供理论依据.To explore the similarity between each sample and develop aquick identification method for CodonopsisRadix,common and variation peaks were calculated as dual-index based on the infrared spectra of Codonopsis Radixfrom different places and the further analysis was used by second derivative infrared spectroscopy.The results indicated that the common peak rates of 4 Codonopsistangshensamples were higher than 71.4%,and 5 Codonopsis pilosula samples were between 55.0% and 83.3%.The highest common peak rate between Codonopsis tangshen and Codonopsis pilosula reached 94.4%,and the lowest rate was 59.1%,while the common peak rates between Codonopsis pilosula var.modestaand Codonopsis tangshen,Codonopsis pilosula were all higher than 65.0%.Therefore,the chemical compositions of Codonopsis Radix maybe have been changed for the changed germplasm resources of Codonopsis Radix from different places.There existed differences between their infrared spectra were more obvious after analyzing through high resolution of second derivative infrared spectroscopy.Fourier transform infrared spectroscopy combined with second derivative infrared spectroscopy could be used to evaluate the rule of the overview of Codonopsis Radixfrom different places and provide a basis for identification and quality control of Codonopsis Radix.

关 键 词：党参 红外指纹图谱 双指标法 二阶导数 化学成分 

分 类 号：R282.5[医药卫生—中药学]