日本结缕草ZjADH基因的克隆及表达分析  

Cloning and Expression of an Alcohol Dehydrogenase Gene(ZjADH) from Zoysia japonica

作　　者：滕珂[1] 李俊[2] 张兰[1] 郭蔚尔 许立新[1] 晁跃辉[1] 

机构地区：[1]北京林业大学林学院草坪研究所,北京100083 [2]中国农业科学院草原研究所,内蒙古呼和浩特010010

出　　处：《中国草地学报》2016年第3期7-13,共7页Chinese Journal of Grassland

基　　金：国家高技术研究发展计划("863"计划)项目"花卉;牧草;竹藤功能基因组学研究与应用"(2013AA102607);中国林学会青年人才托举工程

摘　　要：采用RACE技术从日本结缕草中克隆出1个乙醇脱氢酶基因,命名为ZjADH(GenBank登录号为KT596065)。ZjADH与甘蔗、美洲蒺藜草和沟叶结缕草等ADH基因同源性均在90%以上,进化分析表明其与沟叶结缕草亲缘关系最近。亚细胞定位结果显示,ZjADH定位于细胞质。实时荧光定量分析结果表明,ZjADH在日本结缕草根中表达量最高,ZjADH可响应ABA、MeJA和SA的诱导,在低温、干旱和高盐胁迫中发挥重要作用。The AD H gene,Zj AD H ,was isolated from Zoysia j aponica by RACE method and then submitted to the GenBank （Accession number KT596065 ）. Zj AD H shared a high level of similarity （more than 90% homology）with other AD H genes in Saccharum hybrid ,Cenchrus americanus and Zoy-sia matrella. Phylogenetic analysis showed that ZjADHis was the closest to ZmADH. Subcellular locali-zation of ZjADH was performed by Agrobacterium-mediated transient expression assay in N. benthami-ana. The results demonstrated that ZjADHis localized in the cytoplasm. Quantitative real time PCR was carried out to investigate the expression pattern of Zj AD H. The expression level of Zj AD H was much higher in root,and could be strongly induced by cold,drought or NaCl stresses. Exogenous ABA,MeJA or SA treatment could also up-regulate the expression of Zj AD H as well. Taken together,Zj AD H is a valuable gene in the study of abiotic stresses tolerance of Zoysia j aponica. This study paves the way to further study the Zj AD H in Zoysia j aponica.

关 键 词：乙醇脱氢酶基因 日本结缕草 亚细胞定位 非生物胁迫 激素 

分 类 号：S543.9[农业科学—作物学]