芪黄疽愈方对肢体动脉硬化闭塞症大鼠血脂水平的影响  

Effects of Qihuang-juyu formula on blood liquid in rats with arteriosclerosis obliterans

作　　者：张欣[1] 刘远[1] 葛建立[1] 马云龙[1] 李晓东[2] 张青天 

机构地区：[1]河北省中医院外科,河北石家庄050011 [2]河北省石家庄市中医院脉管二科,河北石家庄050051

出　　处：《河北中医》2016年第5期725-728,共4页Hebei Journal of Traditional Chinese Medicine

基　　金：河北省科学技术厅2014年河北省省级科技计划(第三批)项目(编号:14277760D)

摘　　要：目的观察芪黄疽愈方对肢体动脉硬化闭塞症(ASO)大鼠血脂水平的影响。方法将60只Wistar大鼠随机分为6组,空白组、模型组、西药组及芪黄疽愈方低、中、高剂量组,每组各10只。应用高脂饲料喂饲及隐动脉内膜损伤的方法制作大鼠ASO模型。空白组予普通饮食喂养。模型组予0.9%氯化钠注射液灌胃。芪黄疽愈方低、中、高剂量组均予相应剂量芪黄疽愈方灌胃。西药组予注射用前列地尔干乳剂腹腔注射,西洛他唑片灌胃。除空白组外,其余5组均高脂饲料喂养,并在实验开始时在大鼠右下肢肌肉注射维生素D3注射液。比较各组大鼠血脂水平变化。结果模型组血总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)及极低密度脂蛋白胆固醇(VLDL-C)均较空白组升高(P<0.01),高密度脂蛋白胆固醇(HDL-C)降低(P<0.01),说明造模成功。芪黄疽愈方低剂量组仅TG较模型组降低(P<0.01),芪黄疽愈方中剂量组仅TC、TG较模型组降低(P<0.01),芪黄疽愈方高剂量组、西药组TC、TG、LDL-C及VLDL-C均较模型组降低(P<0.01),HDL-C升高(P<0.01)。芪黄疽愈方高剂量组HDL-C高于西药组(P<0.01)。结论芪黄疽愈方可降低血清TC、TG、LDL-C及VLDL-C,升高HDL-C,通过改善血脂代谢紊乱,达到治疗ASO目的。Objective To observe the effects of Qihuang- juyu formula on blood liquid in rats with arteriosclerosis obliterans（ ASO）. Methods 60 Wistar rats were randomly divided into six groups,that blank group,model group,western medicine group and Qihuang- juyu formula low,middle,high dose group,10 rats in each group. The model of ASO was established by high fat diet feeding and saphenous arterial intimal injury method. Rats in blank group were feed with normal group. Rats in model group were received 0. 9% sodium chloride injection by gavage. Rats in Qihuang- juyu formula low,middle,high dose group were received corresponding concentration of Qihuang- juyu formula by gavage. Rats in western medicine group were received intraperitoneal injection of alprostadil dried emulsion for injection and cilostazol tablets by gavage. Except the blank group,rats in other five groups were feed with high fat diet,and intramuscular injection of vitamin D3 injection on right lower extremity at the beginning of the experiment. The changes of blood liquid were compared. Results The total cholesterol（ TC）,triglyceride（ TC）,low density lipoprotein cholesterol（ LDL- L） and very low density lipoprotein cholesterol（ VLDL- L） in model group were higher than those in blank group（ P〈0. 01）,and high density lipoprotein cholesterol（ HDL- L）was decreased（ P〈0. 01）,which indicated that the ASO model was successful established. Only the TG in low dose group was decreased as compared with that in model group（ P〈0. 01）,only the TC and TG in middle dose group were decreased as compared with those in model group（ P〈0. 01）. The TC,TG,LDL- C and VLDL- C in high dose and western medicine group were decreased as compared with those in model group（ P〈0. 01）,and the HDL- C was increased（ P〈0. 01）. The HDL- C in high dose group was higher than that in western medicine group（ P〈0. 01）. Conclusion Qihuang- juyu formula can decrease TC,TG,LDL- C and VLDL- C,increase HDL-C,improve liquid metabolism diso

关 键 词：动脉硬化 闭塞性 动物 实验 黄芪 黄精 中药疗法 

分 类 号：R323.7[医药卫生—人体解剖和组织胚胎学] R543.505[医药卫生—基础医学]